# User:Allison K. Alix/Notebook/Thesis Research/2013/06/12

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(→Calculating AuNP concentration in g/L) |
(→Dilution of AuNP) |
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==Dilution of AuNP== | ==Dilution of AuNP== | ||

- | According to Reference 1, for the silica coating procedure, AuNP with a radius of ~19nM must | + | According to Reference 1, for the silica coating procedure, 34.7mL AuNP with a radius of ~19nM must have a concentration of 0.058g/L |

M<sub>1</sub>V<sub>1</sub>=M<sub>2</sub>V<sub>2</sub> | M<sub>1</sub>V<sub>1</sub>=M<sub>2</sub>V<sub>2</sub> | ||

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x = 13.37mL in 21.33mL H<sub>2</sub>O | x = 13.37mL in 21.33mL H<sub>2</sub>O | ||

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+ | ==Calculating the amount of PVP to add to AuNP== | ||

## Revision as of 13:06, 12 June 2013

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## AuNP synthesis- Make 20mL AuNP using procedure from User:Allison K. Alix/Notebook/Thesis Research/2013/05/10
- Determine concentration and radius using UV-Vis spectrum
Note: These AuNPs will be used in the silica coating procedure. See calculations below. ## Calculating AuNP diameter and concentration (nM)λ d= [ln(λ where λ d= [ln(527nm-512nm/6.53)]/0.0216
c = A c = 1.89/(4.36x10
## Calculating AuNP concentration in g/L1) Calculate the volume of 1 AuNP Volume of 1 AuNP = 4/3πr V= 4/3π(19.25x10 V= 2.988x10
2) Calculate the mass of 1 AuNP density of Au = 19.3g/cm 19.3g/cm 3) Calculate # of AuNP in 20mL solution 0.43x10 4) Calculate mass of all AuNP in 20mL solution 5.7668x10 ## Dilution of AuNPAccording to Reference 1, for the silica coating procedure, 34.7mL AuNP with a radius of ~19nM must have a concentration of 0.058g/L M (0.1505g/L)(x)=(0.058g/L)(37.4mL) x = 13.37mL in 21.33mL H ## Calculating the amount of PVP to add to AuNP |