User:Allison K. Alix/Notebook/Thesis Research/2013/06/13

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(Autocreate 2013/06/13 Entry for User:Allison_K._Alix/Notebook/Thesis_Research)
Current revision (14:39, 12 July 2013) (view source)
 
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> DNA/MB Sample Prep</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
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==Entry title==
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==MB+DNA Sample Preparation==
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* Insert content here...
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Prepare the following samples for FCS Measurements:
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1) 100nM thiol-DNA + 120nM MB
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2) 1nM DNA + 1.2nM MB
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3) 500pM DNA + 600pM MB
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4) 100pM DNA + 120pM MB
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5) 120nM MB
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6) 1.2nM MB
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7) 600pM MB
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8) 120pM MB
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==Calculations==
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DNA: (100μL)(200nM)= x(53.1μM)
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x= 0.377μL in 99.623μL PBS
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MB: (100μL)(240nM) = x(7.61μM)
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x= 3.154μL in 96.846μL PBS
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1) '''15μL 200nM DNA + 15μL 120nM MB'''
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2) (2.4nm)(30μL) = x(240nM)
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x= 0.3μL 240nM MB in 29.7μL PBS
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(2nM)(30μL) = x(200nM)
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x= 0.3μL 200nM DNA in 29.7μL PBS
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'''15μL 2.4nM MB + 15μL 2nM DNA'''
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3) (1000pM)(30μL)= x (2nM)
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x= 15μL 2nM DNA in 15μL PBS
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(1200pM)(30μL)= x(2.4nmM)
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x= 15μL 2.4nM MB in 15μL PBS
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 +
'''15μL 1000pM DNA + 15μL 1200pM MB'''
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4) (200pM)(30μL) = x(1000pM)
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x= 6μL 1000pM DNA in 24μL PBS
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(240pM)(30μL) = x(1200pM)
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x= 6μL 1200pM DNA in 24μL PBS
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'''15μL 200pM DNA + 15μL 240pM MB'''

Current revision

DNA/MB Sample Prep Main project page
Previous entry      Next entry

MB+DNA Sample Preparation

Prepare the following samples for FCS Measurements:

1) 100nM thiol-DNA + 120nM MB

2) 1nM DNA + 1.2nM MB

3) 500pM DNA + 600pM MB

4) 100pM DNA + 120pM MB

5) 120nM MB

6) 1.2nM MB

7) 600pM MB

8) 120pM MB

Calculations

DNA: (100μL)(200nM)= x(53.1μM)

x= 0.377μL in 99.623μL PBS

MB: (100μL)(240nM) = x(7.61μM)

x= 3.154μL in 96.846μL PBS

1) 15μL 200nM DNA + 15μL 120nM MB

2) (2.4nm)(30μL) = x(240nM)

x= 0.3μL 240nM MB in 29.7μL PBS

(2nM)(30μL) = x(200nM)

x= 0.3μL 200nM DNA in 29.7μL PBS

15μL 2.4nM MB + 15μL 2nM DNA

3) (1000pM)(30μL)= x (2nM)

x= 15μL 2nM DNA in 15μL PBS

(1200pM)(30μL)= x(2.4nmM)

x= 15μL 2.4nM MB in 15μL PBS

15μL 1000pM DNA + 15μL 1200pM MB

4) (200pM)(30μL) = x(1000pM)

x= 6μL 1000pM DNA in 24μL PBS

(240pM)(30μL) = x(1200pM)

x= 6μL 1200pM DNA in 24μL PBS

15μL 200pM DNA + 15μL 240pM MB



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