User:Allison K. Alix/Notebook/Thesis Research/2013/06/13: Difference between revisions
No edit summary |
|||
| (One intermediate revision by the same user not shown) | |||
| Line 1: | Line 1: | ||
{|{{table}} width="800" | {|{{table}} width="800" | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> DNA/MB Sample Prep</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
|- | |- | ||
| Line 36: | Line 36: | ||
x= 3.154μL in 96.846μL PBS | x= 3.154μL in 96.846μL PBS | ||
1) 15μL 200nM DNA + 15μL 120nM MB | 1) '''15μL 200nM DNA + 15μL 120nM MB''' | ||
2) (2.4nm)(30μL) = x(240nM) | 2) (2.4nm)(30μL) = x(240nM) | ||
| Line 46: | Line 46: | ||
x= 0.3μL 200nM DNA in 29.7μL PBS | x= 0.3μL 200nM DNA in 29.7μL PBS | ||
15μL 2.4nM MB + 15μL 2nM DNA | '''15μL 2.4nM MB + 15μL 2nM DNA''' | ||
3) (1000pM)(30μL)= x (2nM) | 3) (1000pM)(30μL)= x (2nM) | ||
| Line 56: | Line 56: | ||
x= 15μL 2.4nM MB in 15μL PBS | x= 15μL 2.4nM MB in 15μL PBS | ||
15μL 1000pM DNA + 15μL 1200pM MB | '''15μL 1000pM DNA + 15μL 1200pM MB''' | ||
4) (200pM)(30μL) = x(1000pM) | 4) (200pM)(30μL) = x(1000pM) | ||
| Line 66: | Line 66: | ||
x= 6μL 1200pM DNA in 24μL PBS | x= 6μL 1200pM DNA in 24μL PBS | ||
15μL 200pM DNA + 15μL 240pM MB | '''15μL 200pM DNA + 15μL 240pM MB''' | ||
Revision as of 11:39, 12 July 2013
 DNA/MB Sample Prep
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
MB+DNA Sample PreparationPrepare the following samples for FCS Measurements: 1) 100nM thiol-DNA + 120nM MB 2) 1nM DNA + 1.2nM MB 3) 500pM DNA + 600pM MB 4) 100pM DNA + 120pM MB 5) 120nM MB 6) 1.2nM MB 7) 600pM MB 8) 120pM MB CalculationsDNA: (100μL)(200nM)= x(53.1μM) x= 0.377μL in 99.623μL PBS MB: (100μL)(240nM) = x(7.61μM) x= 3.154μL in 96.846μL PBS 1) 15μL 200nM DNA + 15μL 120nM MB 2) (2.4nm)(30μL) = x(240nM) x= 0.3μL 240nM MB in 29.7μL PBS (2nM)(30μL) = x(200nM) x= 0.3μL 200nM DNA in 29.7μL PBS 15μL 2.4nM MB + 15μL 2nM DNA 3) (1000pM)(30μL)= x (2nM) x= 15μL 2nM DNA in 15μL PBS (1200pM)(30μL)= x(2.4nmM) x= 15μL 2.4nM MB in 15μL PBS 15μL 1000pM DNA + 15μL 1200pM MB 4) (200pM)(30μL) = x(1000pM) x= 6μL 1000pM DNA in 24μL PBS (240pM)(30μL) = x(1200pM) x= 6μL 1200pM DNA in 24μL PBS 15μL 200pM DNA + 15μL 240pM MB
| |
