# User:Allison K. Alix/Notebook/Thesis Research/2013/06/13

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 Revision as of 11:17, 13 June 2013 (view source) (Autocreate 2013/06/13 Entry for User:Allison_K._Alix/Notebook/Thesis_Research)← Previous diff Revision as of 11:26, 13 June 2013 (view source) (→Entry title)Next diff → Line 6: Line 6: | colspan="2"| | colspan="2"| - ==Entry title== + ==MB+DNA Sample Preparation== - * Insert content here... + + Prepare the following samples for FCS Measurements: + + 1) 100nM thiol-DNA + 120nM MB + + 2) 1nM DNA + 1.2nM MB + + 3) 500pM DNA + 600pM MB + + 4) 100pM DNA + 120pM MB + + 5) 120nM MB + + 6) 1.2nM MB + + 7) 600pM MB + + 8) 120pM MB + + ==Calculations== + + DNA: (100μL)(200nM)= x(53.1μM) + + x= 0.377μL in 99.623μL PBS + + MB: (100μL)(240nM) = x(7.61μM) + + x= 3.154μL in 96.846μL PBS + + 1) 15μL 200nM DNA + 15μL 120nM MB + + 2) (2.4nm)(30μL) = x(240nM) + + x= 0.3μL 240nM MB in 29.7μL PBS + + (2nM)(30μL) = x(200nM) + + x= 0.3μL 200nM DNA in 29.7μL PBS + + 3) (1000pM)(30μL)= x (2nM) + + x= 15μL 2nM DNA in 15μL PBS + + (1200pM)(30μL)= x(2.4nmM) + + x= 15μL 2.4nM MB in 15μL PBS + + 4) (200pM)(30μL) = x(1000pM) + + x= 6μL 1000pM DNA in 24μL PBS + + (240pM)(30μL) = x(1200pM) + + x= 6μL 1200pM DNA in 24μL PBS

## Revision as of 11:26, 13 June 2013

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## MB+DNA Sample Preparation

Prepare the following samples for FCS Measurements:

1) 100nM thiol-DNA + 120nM MB

2) 1nM DNA + 1.2nM MB

3) 500pM DNA + 600pM MB

4) 100pM DNA + 120pM MB

5) 120nM MB

6) 1.2nM MB

7) 600pM MB

8) 120pM MB

## Calculations

DNA: (100μL)(200nM)= x(53.1μM)

x= 0.377μL in 99.623μL PBS

MB: (100μL)(240nM) = x(7.61μM)

x= 3.154μL in 96.846μL PBS

1) 15μL 200nM DNA + 15μL 120nM MB

2) (2.4nm)(30μL) = x(240nM)

x= 0.3μL 240nM MB in 29.7μL PBS

(2nM)(30μL) = x(200nM)

x= 0.3μL 200nM DNA in 29.7μL PBS

3) (1000pM)(30μL)= x (2nM)

x= 15μL 2nM DNA in 15μL PBS

(1200pM)(30μL)= x(2.4nmM)

x= 15μL 2.4nM MB in 15μL PBS

4) (200pM)(30μL) = x(1000pM)

x= 6μL 1000pM DNA in 24μL PBS

(240pM)(30μL) = x(1200pM)

x= 6μL 1200pM DNA in 24μL PBS