User:Allison K. Alix/Notebook/Thesis Research/2013/06/26: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> DNA/MB and AuNP Prep</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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Prepare the following samples: | Prepare the following samples: | ||
1) 100pM DNA | 1) 50nM MB + 40nM DNA | ||
2) 10nM MB + 8nM DNA | |||
3) 5nM MB + 4nM DNA | |||
4) 500pM MB + 400pM DNA | |||
5) 100pM MB + 80pM DNA | |||
6) 50pM MB + 40pM DNA | |||
7) 20pM MB + 16pM DNA | |||
==13.7nm AuNP synthesis== | ==13.7nm AuNP synthesis== | ||
* The nanoparticles produced from this reaction will be used in the silica coating procedure in the future. We are interested in finding a procedure for synthesizing AuNP that has a low diameter variation. The current procedure has a wide range (5-40nm) that greatly affects the amount of PVP/TES that need to be added for the silica coating procedure. Minimizing changes in variables will help simplify the procedure. | |||
1) Add 1mL 0.25mM HAuCl<sub>4</sub> to 100mL water. Stir and heat to boiling. | |||
Current [HAuCl<sub>4</sub>] = 1mM | |||
1mM(x) = (0.25mM)(1mL) | |||
x = 250μL 1mM HAuCl<sub>4</sub> in 750μL H<sub>2</sub>O | |||
2) Add 4.0mL 1% sodium citrate (freshly prepared??) | |||
1g sodium citrate in 100mL of H<sub>2</sub>O | |||
3) Observe a color change to "red wine" | |||
Latest revision as of 22:48, 26 September 2017
DNA/MB and AuNP Prep | Main project page Previous entry Next entry |
Preparation of DNA/MB Samples for FCSPrepare the following samples: 1) 50nM MB + 40nM DNA 2) 10nM MB + 8nM DNA 3) 5nM MB + 4nM DNA 4) 500pM MB + 400pM DNA 5) 100pM MB + 80pM DNA 6) 50pM MB + 40pM DNA 7) 20pM MB + 16pM DNA 13.7nm AuNP synthesis
1) Add 1mL 0.25mM HAuCl4 to 100mL water. Stir and heat to boiling. Current [HAuCl4] = 1mM 1mM(x) = (0.25mM)(1mL) x = 250μL 1mM HAuCl4 in 750μL H2O 2) Add 4.0mL 1% sodium citrate (freshly prepared??) 1g sodium citrate in 100mL of H2O 3) Observe a color change to "red wine"
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