User:Allison K. Alix/Notebook/Thesis Research/2013/06/26

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Preparation of DNA/MB Samples for FCS

Prepare the following samples:

1) 100pM DNA

13.7nm AuNP synthesis

  • The nanoparticles produced from this reaction will be used in the silica coating procedure in the future. We are interested in finding a procedure for synthesizing AuNP that has a low diameter variation. The current procedure has a wide range (5-40nm) that greatly affects the amount of PVP/TES that need to be added for the silica coating procedure. Minimizing changes in variables will help simplify the procedure.

1) Add 1mL 0.25mM HAuCl4 to 100mL water

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