User:Allison K. Alix/Notebook/Thesis Research/2013/06/26
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Preparation of DNA/MB Samples for FCS
Prepare the following samples:
1) 100pM DNA
13.7nm AuNP synthesis
1) Add 1mL 0.25mM HAuCl4 to 100mL water. Stir and heat to boiling.
Current [HAuCl4] = 1mM
1mM(x) = (0.25mM)(1mL)
x = 250μL 1mM HAuCl4 in 750μL H2O
2) Add 4.0mL 1% sodium citrate (freshly prepared??)
1g sodium citrate in 100mL of H2O
3) Observe a color change to "red wine"