User:Allison K. Alix/Notebook/Thesis Research/2013/06/28: Difference between revisions
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==FCS | ==FCS Measurements== | ||
* Run the following samples of MB/DNA | * Run the following samples of MB/DNA | ||
1) | 1) 10nM MB + 8nM DNA | ||
2) | 2) 5nM MB 4nM DNA | ||
3) | 3) 1nM MB 960pM DNA | ||
4) | 4) 500pM MB 400pM DNA | ||
5) | 5) 100pM MB 80pM DNA | ||
6) 25pM MB 20pM DNA | 6) 50pM MB 40pM MB | ||
7) 25pM MB 20pM DNA | |||
* Run the following oligio-D samples | * Run the following oligio-D samples | ||
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5) 100pM | 5) 100pM | ||
==Calculations== | |||
MOLECULAR BEACON: | |||
7.61μM (x) = 20nM (1000uL) | |||
x = 2.62uL 7.61uM MB in 997.38uL PBS | |||
20nM (x) = (30uL)(10nM) | |||
15uL in 15uL PBS | |||
20nM (x) = 2nM(100uL) | |||
10uL in 90uL | |||
2nM (x) = 1nM (100uL) | |||
x = 50uL in 50uL PBS | |||
200pM (100uL) = x (1nM) | |||
x = 20uL in 80uL PBS | |||
100pM(100uL) = (200pM)(x) | |||
x = 50uL in 50uL buffer | |||
50pM (30uL) = 100pM (x) | |||
x = 15uL in 15uL | |||
DNA: | |||
5.31uM (500uL) = x (53.1uM) | |||
x = 50uL in 450uL PBS | |||
5.31uM (x) = 16nM (1000uL) | |||
x = 3uL in 997uL PBS | |||
16nM (x) = (8nM) (100uL) | |||
x = 50uL in 50uL PBS | |||
1.92nM (100uL) = x (8nM) | |||
x = 24uL in 76 uL PBS | |||
0.8nM (100uL) = x (1.92nM) | |||
x = 41.7uL in 58.3uL | |||
160pM (100) = x (800pM) | |||
x = 20uL in 80uL | |||
80pM (100uL) = x (160pM) | |||
x = 50uL in 50uL | |||
40pM (30uL) = x (80pm) | |||
x = 15uL in 15uL PBS | |||
Revision as of 07:43, 28 June 2013
 Project name
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FCS Measurements
1) 10nM MB + 8nM DNA 2) 5nM MB 4nM DNA 3) 1nM MB 960pM DNA 4) 500pM MB 400pM DNA 5) 100pM MB 80pM DNA 6) 50pM MB 40pM MB 7) 25pM MB 20pM DNA
1) 20pM 2) 40pM 3) 60pM 4) 80pM 5) 100pM CalculationsMOLECULAR BEACON: 7.61μM (x) = 20nM (1000uL) x = 2.62uL 7.61uM MB in 997.38uL PBS 20nM (x) = (30uL)(10nM) 15uL in 15uL PBS 20nM (x) = 2nM(100uL) 10uL in 90uL 2nM (x) = 1nM (100uL) x = 50uL in 50uL PBS 200pM (100uL) = x (1nM) x = 20uL in 80uL PBS 100pM(100uL) = (200pM)(x) x = 50uL in 50uL buffer 50pM (30uL) = 100pM (x) x = 15uL in 15uL DNA: 5.31uM (500uL) = x (53.1uM) x = 50uL in 450uL PBS 5.31uM (x) = 16nM (1000uL) x = 3uL in 997uL PBS 16nM (x) = (8nM) (100uL) x = 50uL in 50uL PBS 1.92nM (100uL) = x (8nM) x = 24uL in 76 uL PBS 0.8nM (100uL) = x (1.92nM) x = 41.7uL in 58.3uL 160pM (100) = x (800pM) x = 20uL in 80uL 80pM (100uL) = x (160pM) x = 50uL in 50uL 40pM (30uL) = x (80pm) x = 15uL in 15uL PBS
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