User:Allison K. Alix/Notebook/Thesis Research/2013/07/10: Difference between revisions

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Follow procedure from [[User:Allison_K._Alix/Notebook/Thesis_Research/2013/04/15]]
Follow procedure from [[User:Allison_K._Alix/Notebook/Thesis_Research/2013/04/15]]
Procedures:
1) Mix 250uL 4.405uM DNA with 250uL 100mM DTT in 4% TEA
2) React for 10 minutes
3) Extract DNA using 4 2mL aliquots of ethyl acetate




Revision as of 07:23, 10 July 2013

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DNA-AuNP attachment

  • This will be used to calculate the amount of DNA that is adsorbing onto the gold nanoparticles. I will be able to compare this amount to the amount being adsorbed onto the silica coated gold nanoparticles to get an idea of whether or not the silica hinders that attachment and if so by how much. Will I need to add more DNA?

Follow procedure from User:Allison_K._Alix/Notebook/Thesis_Research/2013/04/15

Procedures:

1) Mix 250uL 4.405uM DNA with 250uL 100mM DTT in 4% TEA

2) React for 10 minutes

3) Extract DNA using 4 2mL aliquots of ethyl acetate




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