User:Allison K. Alix/Notebook/Thesis Research/2013/07/10: Difference between revisions
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3) Extract DNA using 4 2mL aliquots of ethyl acetate | 3) Extract DNA using 4 2mL aliquots of ethyl acetate | ||
4) Add 232.4uL 29.67nM AuNP to 232.4uL 2.22uM DNA | |||
5) React 5 min | |||
6) Add 35.2uL sodium citrate buffer | |||
7) React 10 minutes | |||
8) Centrifuge 3 times for 10000rpm for 3 minutes. Redisperse all supernatants in 50mM HEPES buffer. | |||
9) Take UV-Vis of AuNP/DNA sample and all supernatants. | |||
Revision as of 08:50, 10 July 2013
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DNA-AuNP attachment
Follow procedure from User:Allison_K._Alix/Notebook/Thesis_Research/2013/04/15 Procedures: 1) Mix 250uL 4.405uM DNA with 250uL 100mM DTT in 4% TEA 2) React for 10 minutes 3) Extract DNA using 4 2mL aliquots of ethyl acetate 4) Add 232.4uL 29.67nM AuNP to 232.4uL 2.22uM DNA 5) React 5 min 6) Add 35.2uL sodium citrate buffer 7) React 10 minutes 8) Centrifuge 3 times for 10000rpm for 3 minutes. Redisperse all supernatants in 50mM HEPES buffer. 9) Take UV-Vis of AuNP/DNA sample and all supernatants.
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