User:Allison K. Alix/Notebook/Thesis Research/2013/07/11: Difference between revisions
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x = 50mL in 450mL | x = 50mL in 450mL | ||
==Preparation of 284mM sodium citrate buffer== | |||
[Base] = sodium citrate dihydrate, FW= 294.1 g/mol | |||
[Acid] = citric acid, FW= 210.14 g/mol | |||
pH = pKa + log [base]/[acid] | |||
3.0 = 3.14 + log [base]/[acid] | |||
-0.14 = log [base]/[acid] | |||
[base]/[acid] = 0.7244 | |||
[acid] + [base] = 284mM | |||
[base] = 284mM - [acid] | |||
284mM-[acid]/[acid] = 0.7244 | |||
[acid]= 0.165M | |||
[base]= 0.119M | |||
Revision as of 09:39, 12 July 2013
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To Do
Calculating DNA and AuNP concentrations
x = 1.455uM <-----this is the concentration prior to mixing with AuNP/sodium citrate 1.455uM(500uL) = x (250uL) x = 2.91uM <---this is the concentration prior to mixing with 250uL 100mM DTT in 4% TEA Concentration of stock DNA = 5.31uM 5.31uM(x) = 2.91uM(250uL) x = 137uL in 113uL PBS Preparation of 1X PBSMake 500mL 1X from 10X stock solution 1X(500mL)=(10X)(x) x = 50mL in 450mL Preparation of 284mM sodium citrate buffer[Base] = sodium citrate dihydrate, FW= 294.1 g/mol [Acid] = citric acid, FW= 210.14 g/mol pH = pKa + log [base]/[acid] 3.0 = 3.14 + log [base]/[acid] -0.14 = log [base]/[acid] [base]/[acid] = 0.7244 [acid] + [base] = 284mM [base] = 284mM - [acid] 284mM-[acid]/[acid] = 0.7244 [acid]= 0.165M [base]= 0.119M
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