User:Allison K. Alix/Notebook/Thesis Research/2013/07/15: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 19: Line 19:


* Final concentrations: [AuNP]=14.28nM, [DNA]=1.071uM, [sodium citrate]=10mM
* Final concentrations: [AuNP]=14.28nM, [DNA]=1.071uM, [sodium citrate]=10mM
B) 157.5uL 29.67nM auNP + 157.5uL 2.225uM DNA + 35uL sodium citrate
* Final Concentrations: [AuNP]= 13.35nM, [DNA]=1.0014uM, [sodium citrate]=10mM




Revision as of 07:36, 15 July 2013

DNA/AuNP attachment & Silica Coating Pt 1 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

DNA/AuNP Attachment

1) Prepare 2 solutions containing:

  • 250uL 4.45uM thiol DNA + 250uL 100mM DTT in 4% TEA

2) React 10 minutes and extract DNA using ethyl acetate

3) Prepare the following two solutions

A) 240.7uL 29.67nM AuNP + 240.7uL 2.225uM DNA + 18.7uL sodium citrate

  • Final concentrations: [AuNP]=14.28nM, [DNA]=1.071uM, [sodium citrate]=10mM

B) 157.5uL 29.67nM auNP + 157.5uL 2.225uM DNA + 35uL sodium citrate

  • Final Concentrations: [AuNP]= 13.35nM, [DNA]=1.0014uM, [sodium citrate]=10mM







Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Allison_K._Alix/Notebook/Thesis_Research/2013/07/15&oldid=709181"