User:Allison K. Alix/Notebook/Thesis Research/2013/07/15: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 27: Line 27:


5) Centrifuge for 4 minutes at 7000rpm, redisperse in 50mM HEPES buffer
5) Centrifuge for 4 minutes at 7000rpm, redisperse in 50mM HEPES buffer
==Silica Coating Procedure: Part 1==
* Prior to addition of TES to AuNP, MHA must be attached to AuNP
1) Dissolve 0.0144g MHA in 5mL PBS, combine with 5mL AuNPs
2) Stir overnight




Revision as of 07:40, 15 July 2013

DNA/AuNP attachment & Silica Coating Pt 1 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

DNA/AuNP Attachment

1) Prepare 2 solutions containing:

  • 250uL 4.45uM thiol DNA + 250uL 100mM DTT in 4% TEA

2) React 10 minutes and extract DNA using ethyl acetate

3) Prepare the following two solutions

A) 240.7uL 29.67nM AuNP + 240.7uL 2.225uM DNA + 18.7uL sodium citrate

  • Final concentrations: [AuNP]=14.28nM, [DNA]=1.071uM, [sodium citrate]=10mM

B) 157.5uL 29.67nM auNP + 157.5uL 2.225uM DNA + 35uL sodium citrate

  • Final Concentrations: [AuNP]= 13.35nM, [DNA]=1.0014uM, [sodium citrate]=10mM

4) React 10 minutes

5) Centrifuge for 4 minutes at 7000rpm, redisperse in 50mM HEPES buffer

Silica Coating Procedure: Part 1

  • Prior to addition of TES to AuNP, MHA must be attached to AuNP

1) Dissolve 0.0144g MHA in 5mL PBS, combine with 5mL AuNPs

2) Stir overnight







Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Allison_K._Alix/Notebook/Thesis_Research/2013/07/15&oldid=709185"