User:Allison K. Alix/Notebook/Thesis Research/2013/07/16: Difference between revisions
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Surface Area of a sphere = 4πr<sup>2</sup>, r = 5nm | Surface Area of a sphere = 4πr<sup>2</sup>, r = 5nm | ||
4π(5x10<sup>-9</sup>m)<sup>2</sup> = 3.14 x 10<sup>-16</sup>m<sup>2</sup> = 314nm<sup>2</sup> | 4π(5x10<sup>-9</sup>m)<sup>2</sup> = 3.14 x 10<sup>-16</sup>m<sup>2</sup> = '''314nm<sup>2</sup>''' | ||
'''Calculating the # of AuNP in 240.7μL 29.67nM solution''' | '''Calculating the # of AuNP in 240.7μL 29.67nM solution''' | ||
29.67 x 10<sup>-9</sup>mol/L x 240 x 10<sup>-6</sup>L x 6.02x10<sup>23</sup> AuNP/mol = 4.299 x 10<sup>12</sup> AuNP | 29.67 x 10<sup>-9</sup>mol/L x 240 x 10<sup>-6</sup>L x 6.02x10<sup>23</sup> AuNP/mol = '''4.299 x 10<sup>12</sup> AuNP''' | ||
'''Calculating the Total Surface Area Available for DNA Attachment''' | |||
4.299 x 10<sup>12</sup> AuNP x 314nm<sup>2</sup>/1 AuNP = '''1.349886 x 10<sup>15</sup>nm<sup>2</sup>''' | |||
Revision as of 11:11, 16 July 2013
UV-Vis Analysis/Silica Coating Pt. 2 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
To Do
Silica Coating Procedure1) Prepare the following solutions:
3) Centrifuge solutions at 4000 rpm for one hour 4) Re-suspend particles in PBS to a final concentration of 5nM? DNA/AuNP Data AnalysisSee File:2013 0715 AuNP-DNA.xlsx for AuNP/DNA spectra as well as absorbance values and molar extinction coefficients used to calculate concentrations of each in final solutions. Calculating the surface area of 1 AuNP Surface Area of a sphere = 4πr2, r = 5nm 4π(5x10-9m)2 = 3.14 x 10-16m2 = 314nm2 Calculating the # of AuNP in 240.7μL 29.67nM solution 29.67 x 10-9mol/L x 240 x 10-6L x 6.02x1023 AuNP/mol = 4.299 x 1012 AuNP Calculating the Total Surface Area Available for DNA Attachment 4.299 x 1012 AuNP x 314nm2/1 AuNP = 1.349886 x 1015nm2
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