User:Allison K. Alix/Notebook/Thesis Research/2013/07/16
From OpenWetWare
(→DNA/AuNP Data Analysis) 
(→DNA/AuNP Data Analysis) 

Line 63:  Line 63:  
Sample A1  Sample A1  
  +  moles DNA = (1.827x10<sup>7</sup>M x 350 x 10<sup>6</sup>L) = 6.40x10<sup>11</sup> moles  
+  
+  % hybridized = (6.40x10<sup>11</sup>/5.3555575 x 10<sup>10</sup> moles DNA)x100% = 11.95%  
+  
Revision as of 13:37, 16 July 2013
UVVis Analysis/Silica Coating Pt. 2  Main project page Previous entry Next entry 
To Do
Silica Coating Procedure1) Prepare the following solutions:
3) Centrifuge solutions at 4000 rpm for one hour 4) Resuspend particles in PBS to a final concentration of 5nM? DNA/AuNP Data AnalysisSee Image:2013 0715 AuNPDNA.xlsx for AuNP/DNA spectra as well as absorbance values and molar extinction coefficients used to calculate concentrations of each in final solutions. Calculating the surface area of 1 AuNP Surface Area of a sphere = 4πr^{2}, r = 5nm 4π(5x10^{9}m)^{2} = 3.14 x 10^{16}m^{2} = 314nm^{2} Calculating the # of AuNP in 240.7μL 29.67nM solution 29.67 x 10^{9}mol/L x 240 x 10^{6}L x 6.02x10^{23} AuNP/mol = 4.299 x 10^{12} AuNP Calculating the Total Surface Area Available for DNA Attachment 4.299 x 10^{12} AuNP x 3.14x10^{16}m^{2}/1 AuNP = 0.001349886 m^{2} Calculating the Number of Moles of DNA Available to Attach 2.225x10^{6}moles/L x 240.7x10^{6}L = 5.3555575 x 10^{10} moles DNA Calculating oligonucleotide density on each particle 5.3555575x10^{10}moles DNA/0.001349886m^{2} AuNP = 3.967x10^{7} moles DNA/m^{2} AuNP (OR 39.67pmol DNA/cm^{2} AuNP) Calculating the percent DNA hybridized in each sample
Sample A1 moles DNA = (1.827x10^{7}M x 350 x 10^{6}L) = 6.40x10^{11} moles % hybridized = (6.40x10^{11}/5.3555575 x 10^{10} moles DNA)x100% = 11.95%
