User:Allison K. Alix/Notebook/Thesis Research/2013/07/16: Difference between revisions
From OpenWetWare
| Line 34: | Line 34: | ||
See [[Image:2013_0715_AuNP-DNA.xlsx]] for AuNP/DNA spectra as well as absorbance values and molar extinction coefficients used to calculate concentrations of each in final solutions. | See [[Image:2013_0715_AuNP-DNA.xlsx]] for AuNP/DNA spectra as well as absorbance values and molar extinction coefficients used to calculate concentrations of each in final solutions. | ||
'''Calculating the surface area of 1 AuNP''' | |||
Surface Area of a sphere = 4πr<sup>2</sup>, r = 5nm | |||
4π(5x10<sup>-9</sup>m)<sup>2</sup> = 3.14 x 10<sup>-16</sup>m<sup>2</sup> = 314nm<sup>2</sup> | |||
Revision as of 11:06, 16 July 2013
 UV-Vis Analysis/Silica Coating Pt. 2
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
To Do
Silica Coating Procedure1) Prepare the following solutions:
3) Centrifuge solutions at 4000 rpm for one hour 4) Re-suspend particles in PBS to a final concentration of 5nM? UV-Vis Data AnalysisSee File:2013 0715 AuNP-DNA.xlsx for AuNP/DNA spectra as well as absorbance values and molar extinction coefficients used to calculate concentrations of each in final solutions. Calculating the surface area of 1 AuNP Surface Area of a sphere = 4πr2, r = 5nm 4π(5x10-9m)2 = 3.14 x 10-16m2 = 314nm2
| |
