User:Allison K. Alix/Notebook/Thesis Research/2013/07/16: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> UV-Vis Analysis/Silica Coating Pt. 2</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> UV-Vis Analysis/Silica Coating Pt. 2</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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4π(5x10<sup>-9</sup>m)<sup>2</sup> = 3.14 x 10<sup>-16</sup>m<sup>2</sup> = '''314nm<sup>2</sup>''' | 4π(5x10<sup>-9</sup>m)<sup>2</sup> = 3.14 x 10<sup>-16</sup>m<sup>2</sup> = '''314nm<sup>2</sup>''' | ||
'''Calculating the # of AuNP in 240.7μL 29.67nM solution''' | '''Calculating the # of AuNP in 240.7μL 29.67nM solution''' | ||
29.67 x 10<sup>-9</sup>mol/L x 240 x 10<sup>-6</sup>L x 6.02x10<sup>23</sup> AuNP/mol = '''4.299 x 10<sup>12</sup> AuNP''' | 29.67 x 10<sup>-9</sup>mol/L x 240 x 10<sup>-6</sup>L x 6.02x10<sup>23</sup> AuNP/mol = '''4.299 x 10<sup>12</sup> AuNP''' | ||
'''Calculating the Total Surface Area Available for DNA Attachment''' | '''Calculating the Total Surface Area Available for DNA Attachment''' | ||
4.299 x 10<sup>12</sup> AuNP x | 4.299 x 10<sup>12</sup> AuNP x 3.14x10<sup>-16</sup>m<sup>2</sup>/1 AuNP = '''0.001349886 m<sup>2</sup>''' | ||
'''Calculating the Number of Moles of DNA Available to Attach''' | '''Calculating the Number of Moles of DNA Available to Attach''' | ||
2.225x10<sup>-6</sup>moles/L x 240.7x10<sup>-6</sup>L = '''5.3555575 x 10<sup>-10</sup> moles DNA''' | 2.225x10<sup>-6</sup>moles/L x 240.7x10<sup>-6</sup>L = '''5.3555575 x 10<sup>-10</sup> moles DNA''' | ||
'''Calculating oligonucleotide density on each particle (theoretical)''' | |||
5.3555575x10<sup>-10</sup>moles DNA/0.001349886m<sup>2</sup> AuNP = '''3.967x10<sup>-7</sup> moles DNA/m<sup>2</sup> AuNP''' (OR 39.67pmol DNA/cm<sup>2</sup> AuNP) | |||
'''Calculating oligonucleotide density on each particle (actual)''' | |||
Sample A1 | |||
moles DNA = (1.827x10<sup>-7</sup>M x 350 x 10<sup>-6</sup>L) = 6.40x10<sup>-11</sup> moles | |||
6.40x10<sup>-11</sup> moles/0.001349886m<sup>2</sup> AuNP = '''4.74x10<sup>-8</sup>moles DNA/m<sup>2</sup>AuNP''' | |||
Sample B | |||
moles DNA = 4.44x10<sup>-8</sup>M x 500 x 10<sup>-6</sup>L = 2.22x10<sup>-11</sup> moles | |||
2.22x10<sup>-11</sup> moles/0.001349886m<sup>2</sup> AuNP = '''1.64x10<sup>-8</sup>moles DNA/m<sup>2</sup>AuNP''' | |||
Sample A2 | |||
moles DNA = 1.42x10<sup>-10</sup> | |||
1.42x10<sup>-10</sup>/0.001349886m<sup>2</sup> AuNP = '''1.05x10<sup>-7</sup>moles DNA/m<sup>2</sup>AuNP''' | |||
'''Calculating the percentage of DNA hybridized in each sample''' | |||
Sample A1 | |||
4.74x10<sup>-8</sup>moles DNA/m<sup>2</sup>AuNP/3.967x10<sup>-7</sup> moles DNA/m<sup>2</sup> AuNP = '''11.95%''' | |||
Sample B | |||
1.64x10<sup>-8</sup>moles DNA/m<sup>2</sup>AuNP/3.967x10<sup>-7</sup> moles DNA/m<sup>2</sup> AuNP = '''4.12%''' | |||
Sample A2 | |||
1.05x10<sup>-7</sup>moles DNA/m<sup>2</sup>AuNP/3.967x10<sup>-7</sup> moles DNA/m<sup>2</sup> AuNP = '''26.5%''' | |||
==References== | |||
[https://www.cs.duke.edu/~reif/temp/IN.VITRO.EVO/OTHER/DNA%20AuNP/Taton%20Protocol.pdf], [http://pubs.acs.org/doi/pdf/10.1021/ac0006627] | |||
Latest revision as of 23:04, 26 September 2017
UV-Vis Analysis/Silica Coating Pt. 2 | Main project page Previous entry Next entry |
To Do
Silica Coating Procedure1) Prepare the following solutions:
3) Centrifuge solutions at 4000 rpm for one hour 4) Re-suspend particles in PBS to a final concentration of 5nM? DNA/AuNP Data AnalysisSee File:2013 0715 AuNP-DNA.xlsx for AuNP/DNA spectra as well as absorbance values and molar extinction coefficients used to calculate concentrations of each in final solutions. Calculating the surface area of 1 AuNP Surface Area of a sphere = 4πr2, r = 5nm 4π(5x10-9m)2 = 3.14 x 10-16m2 = 314nm2
29.67 x 10-9mol/L x 240 x 10-6L x 6.02x1023 AuNP/mol = 4.299 x 1012 AuNP
4.299 x 1012 AuNP x 3.14x10-16m2/1 AuNP = 0.001349886 m2
2.225x10-6moles/L x 240.7x10-6L = 5.3555575 x 10-10 moles DNA
5.3555575x10-10moles DNA/0.001349886m2 AuNP = 3.967x10-7 moles DNA/m2 AuNP (OR 39.67pmol DNA/cm2 AuNP)
Sample A1 moles DNA = (1.827x10-7M x 350 x 10-6L) = 6.40x10-11 moles 6.40x10-11 moles/0.001349886m2 AuNP = 4.74x10-8moles DNA/m2AuNP Sample B moles DNA = 4.44x10-8M x 500 x 10-6L = 2.22x10-11 moles 2.22x10-11 moles/0.001349886m2 AuNP = 1.64x10-8moles DNA/m2AuNP Sample A2 moles DNA = 1.42x10-10 1.42x10-10/0.001349886m2 AuNP = 1.05x10-7moles DNA/m2AuNP
Sample A1 4.74x10-8moles DNA/m2AuNP/3.967x10-7 moles DNA/m2 AuNP = 11.95% Sample B 1.64x10-8moles DNA/m2AuNP/3.967x10-7 moles DNA/m2 AuNP = 4.12% Sample A2 1.05x10-7moles DNA/m2AuNP/3.967x10-7 moles DNA/m2 AuNP = 26.5% References
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