User:Allison K. Alix/Notebook/Thesis Research/2013/07/16: Difference between revisions
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2.225x10<sup>-6</sup>moles/L x 240.7x10<sup>-6</sup>L = '''5.3555575 x 10<sup>-10</sup> moles DNA''' | 2.225x10<sup>-6</sup>moles/L x 240.7x10<sup>-6</sup>L = '''5.3555575 x 10<sup>-10</sup> moles DNA''' | ||
'''Calculating oligonucleotide density on each particle''' | '''Calculating oligonucleotide density on each particle (theoretical)''' | ||
5.3555575x10<sup>-10</sup>moles DNA/0.001349886m<sup>2</sup> AuNP = '''3.967x10<sup>-7</sup> moles DNA/m<sup>2</sup> AuNP''' (OR 39.67pmol DNA/cm<sup>2</sup> AuNP) | 5.3555575x10<sup>-10</sup>moles DNA/0.001349886m<sup>2</sup> AuNP = '''3.967x10<sup>-7</sup> moles DNA/m<sup>2</sup> AuNP''' (OR 39.67pmol DNA/cm<sup>2</sup> AuNP) | ||
'''Calculating oligonucleotide density on each particle (actual)''' | |||
Sample A1 | |||
moles DNA = (1.827x10<sup>-7</sup>M x 350 x 10<sup>-6</sup>L) = 6.40x10<sup>-11</sup> moles | |||
6.40x10<sup>-11</sup> moles/0.001349886m<sup>2</sup> AuNP = 4.74x10<sup>-8</sup>moles DNA/m<sup>2</sup>AuNP | |||
Sample B | |||
moles DNA = 4.44x10<sup>-8</sup>M x 500 x 10<sup>-6</sup>L = 2.22x10<sup>-11</sup> moles | |||
2.22x10<sup>-11</sup> moles/0.001349886m<sup>2</sup> AuNP = 1.64x10<sup>-8</sup>moles DNA/m<sup>2</sup>AuNP | |||
Sample A2 | |||
moles DNA = 1.42x10<sup>-10</sup> | |||
1.42x10<sup>-10</sup>/0.001349886m<sup>2</sup> AuNP = 1.05x10<sup>-7</sup>moles DNA/m<sup>2</sup>AuNP | |||
'''Calculating the percentage of DNA hybridized in each sample''' | '''Calculating the percentage of DNA hybridized in each sample''' | ||
Revision as of 11:52, 16 July 2013
 UV-Vis Analysis/Silica Coating Pt. 2
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To Do
Silica Coating Procedure1) Prepare the following solutions:
3) Centrifuge solutions at 4000 rpm for one hour 4) Re-suspend particles in PBS to a final concentration of 5nM? DNA/AuNP Data AnalysisSee File:2013 0715 AuNP-DNA.xlsx for AuNP/DNA spectra as well as absorbance values and molar extinction coefficients used to calculate concentrations of each in final solutions. Calculating the surface area of 1 AuNP Surface Area of a sphere = 4πr2, r = 5nm 4π(5x10-9m)2 = 3.14 x 10-16m2 = 314nm2 Calculating the # of AuNP in 240.7μL 29.67nM solution 29.67 x 10-9mol/L x 240 x 10-6L x 6.02x1023 AuNP/mol = 4.299 x 1012 AuNP Calculating the Total Surface Area Available for DNA Attachment 4.299 x 1012 AuNP x 3.14x10-16m2/1 AuNP = 0.001349886 m2 Calculating the Number of Moles of DNA Available to Attach 2.225x10-6moles/L x 240.7x10-6L = 5.3555575 x 10-10 moles DNA Calculating oligonucleotide density on each particle (theoretical) 5.3555575x10-10moles DNA/0.001349886m2 AuNP = 3.967x10-7 moles DNA/m2 AuNP (OR 39.67pmol DNA/cm2 AuNP) Calculating oligonucleotide density on each particle (actual) Sample A1 moles DNA = (1.827x10-7M x 350 x 10-6L) = 6.40x10-11 moles 6.40x10-11 moles/0.001349886m2 AuNP = 4.74x10-8moles DNA/m2AuNP Sample B moles DNA = 4.44x10-8M x 500 x 10-6L = 2.22x10-11 moles 2.22x10-11 moles/0.001349886m2 AuNP = 1.64x10-8moles DNA/m2AuNP Sample A2 moles DNA = 1.42x10-10 1.42x10-10/0.001349886m2 AuNP = 1.05x10-7moles DNA/m2AuNP
Sample A1 moles DNA = (1.827x10-7M x 350 x 10-6L) = 6.40x10-11 moles % hybridized = (6.40x10-11/5.3555575 x 10-10 moles DNA)x100% = 11.95% Sample B moles DNA = 4.44x10-8M x 500 x 10-6L = 2.22x10-11 moles % hybridized = (2.22x10-11 moles/5.3555575 x 10-10 moles DNA)x100% = 4.14% Sample A2 moles DNA = 1.42x10-10 % hybridized = (1.42x10-10/5.3555575 x 10-10) = 26.5%
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