User:Allison K. Alix/Notebook/Thesis Research/2013/07/17: Difference between revisions

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* Obtain UV-Vis Data for DNA/AuNP samples and perform calculations following steps from [[User:Allison K. Alix/Notebook/Thesis Research/2013/07/16]]
* Obtain UV-Vis Data for DNA/AuNP samples and perform calculations following steps from [[User:Allison K. Alix/Notebook/Thesis Research/2013/07/16]]
==DNA-AuNP attachment procedure==
*Scale down to a final solution volume of 300uL due to minimal AuNP solution.
'''Calculating volumes of buffer, AuNP, and DNA for final solution:'''
[sodium citrate] = 268mM
268mM (x) = 10mM (300uL)
x = 11.2uL sodium citrate
(300uL-11.2uL)/2 = 144.4uL each of DNA and AuNP
* Prepare




Revision as of 06:58, 17 July 2013

Absorbance Measurements of AuNP@SiO2 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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To Do

  • Take absorbance spectra of AuNP@SiO2 samples
  • Determine which concentrations are suitable for TEM imaging (Thursday)
  • Repeat DNA/AuNP extraction procedure using optimum centrifugation speed and time (10,000 rpm for 11 minutes)

DNA-AuNP attachment procedure

  • Scale down to a final solution volume of 300uL due to minimal AuNP solution.

Calculating volumes of buffer, AuNP, and DNA for final solution:

[sodium citrate] = 268mM

268mM (x) = 10mM (300uL)

x = 11.2uL sodium citrate

(300uL-11.2uL)/2 = 144.4uL each of DNA and AuNP

  • Prepare






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