# User:Allison K. Alix/Notebook/Thesis Research/2013/07/17

(Difference between revisions)
 Revision as of 15:07, 16 July 2013 (view source)← Previous diff Revision as of 09:58, 17 July 2013 (view source) (→To Do)Next diff → Line 15: Line 15: * Obtain UV-Vis Data for DNA/AuNP samples and perform calculations following steps from [[User:Allison K. Alix/Notebook/Thesis Research/2013/07/16]] * Obtain UV-Vis Data for DNA/AuNP samples and perform calculations following steps from [[User:Allison K. Alix/Notebook/Thesis Research/2013/07/16]] + + ==DNA-AuNP attachment procedure== + + *Scale down to a final solution volume of 300uL due to minimal AuNP solution. + + '''Calculating volumes of buffer, AuNP, and DNA for final solution:''' + + [sodium citrate] = 268mM + + 268mM (x) = 10mM (300uL) + + x = 11.2uL sodium citrate + + (300uL-11.2uL)/2 = 144.4uL each of DNA and AuNP + + * Prepare + + + + + + +

## Revision as of 09:58, 17 July 2013

Absorbance Measurements of AuNP@SiO2 Main project page
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## To Do

• Take absorbance spectra of AuNP@SiO2 samples
• Determine which concentrations are suitable for TEM imaging (Thursday)
• Repeat DNA/AuNP extraction procedure using optimum centrifugation speed and time (10,000 rpm for 11 minutes)

## DNA-AuNP attachment procedure

• Scale down to a final solution volume of 300uL due to minimal AuNP solution.

Calculating volumes of buffer, AuNP, and DNA for final solution:

[sodium citrate] = 268mM

268mM (x) = 10mM (300uL)

x = 11.2uL sodium citrate

(300uL-11.2uL)/2 = 144.4uL each of DNA and AuNP

• Prepare