# User:Allison K. Alix/Notebook/Thesis Research/2013/07/18

(Difference between revisions)
 Revision as of 11:11, 19 July 2013 (view source) (→%DNA hybridized on AuNP from 7/16)← Previous diff Revision as of 11:17, 19 July 2013 (view source) (→%DNA hybridized on AuNP from 7/16)Next diff → Line 17: Line 17: 29.67 x 10-9mol/L x 144.4x10-6L x 6.02x1023 AuNP/mol = 2.579x1012 AuNP 29.67 x 10-9mol/L x 144.4x10-6L x 6.02x1023 AuNP/mol = 2.579x1012 AuNP + '''Calculating the Total Surface Area Available for DNA Attachment''' '''Calculating the Total Surface Area Available for DNA Attachment''' 2.579x1012AuNP x 3.14x10-16m2/1 AuNP = 0.00081 m2 2.579x1012AuNP x 3.14x10-16m2/1 AuNP = 0.00081 m2 + '''Calculating the Number of Moles of DNA Available to Attach''' '''Calculating the Number of Moles of DNA Available to Attach''' Line 26: Line 28: 2.225x10-6moles/L x 144.4x10-6L = 3.2129x10-10 moles 2.225x10-6moles/L x 144.4x10-6L = 3.2129x10-10 moles - Calculating oligonucleotide density on each particle (theoretical) + + '''Calculating oligonucleotide density on each particle (theoretical)''' 3.2129x10-10 moles/0.00081 m2 AuNP = 3.967x10-7 moles DNA/m2 AuNP (OR 39.67pmol DNA/cm2 AuNP) 3.2129x10-10 moles/0.00081 m2 AuNP = 3.967x10-7 moles DNA/m2 AuNP (OR 39.67pmol DNA/cm2 AuNP) - Calculating oligonucleotide density on each particle (actual) - Sample A1 + '''Calculating oligonucleotide density on each particle (actual)''' + + moles DNA = (1.16872x10-7M x 300 x 10-6L) = 3.51x10-11 moles + + 3.51x10-11 moles/0.00081 m2 AuNP = 4.33x10-8moles DNA/m2AuNP + + + '''Calculating the percentage of DNA hybridized in each sample''' - moles DNA = (1.827x10-7M x 350 x 10-6L) = 6.40x10-11 moles + 4.33x10-8moles DNA/m2AuNP/3.967x10-7 moles DNA/m2 AuNP = 10.9% - 6.40x10-11 moles/0.001349886m2 AuNP = 4.74x10-8moles DNA/m2AuNP + - Sample B + - moles DNA = 4.44x10-8M x 500 x 10-6L = 2.22x10-11 moles + - 2.22x10-11 moles/0.001349886m2 AuNP = 1.64x10-8moles DNA/m2AuNP + - Sample A2 + - moles DNA = 1.42x10-10 + - 1.42x10-10/0.001349886m2 AuNP = 1.05x10-7moles DNA/m2AuNP + - Calculating the percentage of DNA hybridized in each sample - Sample A1 - 4.74x10-8moles DNA/m2AuNP/3.967x10-7 moles DNA/m2 AuNP = 11.95% - Sample B - 1.64x10-8moles DNA/m2AuNP/3.967x10-7 moles DNA/m2 AuNP = 4.12% - Sample A2 - 1.05x10-7moles DNA/m2AuNP/3.967x10-7 moles DNA/m2 AuNP = 26.5%

## Revision as of 11:17, 19 July 2013

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## %DNA hybridized on AuNP from 7/16

Calculating the surface area of 1 AuNP

Surface Area of a sphere = 4πr2, r = 5nm

4π(5x10-9m)2 = 3.14 x 10-16m2 = 314nm2

Calculating the # of AuNP in 144.4uL 29.67nM solution

29.67 x 10-9mol/L x 144.4x10-6L x 6.02x1023 AuNP/mol = 2.579x1012 AuNP

Calculating the Total Surface Area Available for DNA Attachment

2.579x1012AuNP x 3.14x10-16m2/1 AuNP = 0.00081 m2

Calculating the Number of Moles of DNA Available to Attach

2.225x10-6moles/L x 144.4x10-6L = 3.2129x10-10 moles

Calculating oligonucleotide density on each particle (theoretical)

3.2129x10-10 moles/0.00081 m2 AuNP = 3.967x10-7 moles DNA/m2 AuNP (OR 39.67pmol DNA/cm2 AuNP)

Calculating oligonucleotide density on each particle (actual)

moles DNA = (1.16872x10-7M x 300 x 10-6L) = 3.51x10-11 moles

3.51x10-11 moles/0.00081 m2 AuNP = 4.33x10-8moles DNA/m2AuNP

Calculating the percentage of DNA hybridized in each sample

4.33x10-8moles DNA/m2AuNP/3.967x10-7 moles DNA/m2 AuNP = 10.9%