User:Allison K. Alix/Notebook/Thesis Research/2013/07/19: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Synthesis of AuNP</span> | ||
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== | ==AuNP synthesis procedures== | ||
* | |||
* We will perform three procedures and observe which produces the desired AuNP diameter (~10nm) | |||
'''Procedure 1''' Taken from [[User:Allison K. Alix/Notebook/Thesis Research/2013/05/10]] | |||
1) Stir and heat to boiling 20mL of 1mM HAuCl4 | |||
2) Add 2mL 1% sodium citrate | |||
3) Observe a purple to red color change | |||
4) Determine size and concentration of AuNP using UV-Vis | |||
'''Procedure 2'''[http://pubs.acs.org/doi/pdf/10.1021/la034348u] | |||
1) Boil 50mL MilliQ water in a clean beaker | |||
2) Add 0.5 mL of HAuCl4 solution (1% w/v) | |||
*1% w/v = (0.01g in 1mL water) | |||
3) Follow with 1.75 mL of sodium citrate solution (1% w/v) | |||
4) Boil for about 10 minutes until solution color changes to bright red | |||
5) Replace water lost to evaporation during boiling | |||
6) If a higher concentration is desired, centrifuge at 14 000 rpm and resuspended in MilliQ water | |||
'''Procedure 3'''[http://pubs.acs.org/doi/pdf/10.1021/ac0503198] | |||
1) Bring 100 mL of solution containing 0.01 g of HAuCl4·3H2O to reflux | |||
2) Add 3 mL of 1% sodium citrate solution while stirring | |||
3) Boil solution for another 40 minutes | |||
4) Cool to room temperature | |||
* Obtain UV-Vis Spectra of all AuNP | |||
==Fluorescent changes based on number of G-quadruplexs formed== | |||
* We have already seen that increasing the DNA concentration of an 8-repeat strand results in an increased fluorescence signal. But what happens when we keep concentration of DNA constant and instead decrease the number of repeats to 4, so that only 1 G-quadruplex is formed? Although it was seen that this trend was true using ZnPPIX as a G-quadruplex inducer and fluorescent sensor, it would be beneficial for this research to examine if the same was true for ThT. This would support the idea that the number of G-quadruplexes can be determined based on fluorescent measurements, both bulk and FCS. | |||
Revision as of 09:05, 19 July 2013
 Synthesis of AuNP
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AuNP synthesis procedures
Procedure 1 Taken from User:Allison K. Alix/Notebook/Thesis Research/2013/05/10 1) Stir and heat to boiling 20mL of 1mM HAuCl4 2) Add 2mL 1% sodium citrate 3) Observe a purple to red color change 4) Determine size and concentration of AuNP using UV-Vis Procedure 2[1] 1) Boil 50mL MilliQ water in a clean beaker 2) Add 0.5 mL of HAuCl4 solution (1% w/v)
3) Follow with 1.75 mL of sodium citrate solution (1% w/v) 4) Boil for about 10 minutes until solution color changes to bright red 5) Replace water lost to evaporation during boiling 6) If a higher concentration is desired, centrifuge at 14 000 rpm and resuspended in MilliQ water Procedure 3[2] 1) Bring 100 mL of solution containing 0.01 g of HAuCl4·3H2O to reflux 2) Add 3 mL of 1% sodium citrate solution while stirring 3) Boil solution for another 40 minutes 4) Cool to room temperature
Fluorescent changes based on number of G-quadruplexs formed
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