User:Allison K. Alix/Notebook/Thesis Research/2013/07/25: Difference between revisions
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'''4-repeat''' | '''4-repeat''' | ||
x(500uL)=(265.6uM)( | x(500uL)=(265.6uM)(10) | ||
x = | x = 5.31uM | ||
[100-([x(100)]/5.31)] = | |||
1800nM--> 33.9uL in 58.2uL PBS add 7.9 | |||
1400nM--> 26.4uL in 67.5uL add 6.1 | |||
1000nM--> 18.8uL in 76.8uL add 4.4 | |||
200nM--> | 600nM--> 11.3uL in 86.1uL add 2.6 | ||
200nM--> 3.8uL in 95.4uL add 0.8 | |||
'''8-repeat''' | '''8-repeat''' |
Revision as of 09:01, 25 July 2013
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To Do
Silica-Coating Part 11) Dilute 23.5nM AuNPs to 9.4nM (2mL AuNPs in 3mL water *NOTE: the water was left to assimilate to room temperature prior to dilution*) 2) ThT/DNA attachment1) Prepare 100uL of the following concentrations of 4-strand and 8-strand telomeric DNA (which should generate 1 and 2 G-quadruplexes respectively) a) 100nM b) 300nM c) 500nM d) 700nM e) 900nM NOTE: Original Concentrations of 4 and 8-repeats are 265.6uM and 142.8uM, respectively 4-repeat x(500uL)=(265.6uM)(10) x = 5.31uM [100-([x(100)]/5.31)] = 1800nM--> 33.9uL in 58.2uL PBS add 7.9 1400nM--> 26.4uL in 67.5uL add 6.1 1000nM--> 18.8uL in 76.8uL add 4.4 600nM--> 11.3uL in 86.1uL add 2.6 200nM--> 3.8uL in 95.4uL add 0.8 8-repeat 142.8uM(10uL) = (x)(500uL) x= 2.856uM 1800nM--> 63uL in 37uL PBS 1400nM--> 49uL in 51uL 1000nM--> 35uL in 65uL 600nM--> 21uL in 79uL 200nM--> 7uL in 93uL
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