User:Allison K. Alix/Notebook/Thesis Research/2013/07/25

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(To Do)
(Silica-Coating Part 1)
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==Silica-Coating Part 1==
==Silica-Coating Part 1==
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1) Dilute 23.5nM AuNPs to 9.4nM (2mL AuNPs in 3mL water *NOTE: the water was left to assimilate to room temperature*)
+
1) Dilute 23.5nM AuNPs to 9.4nM (2mL AuNPs in 3mL water *NOTE: the water was left to assimilate to room temperature prior to dilution*)
 +
 
 +
2)
 +
 
 +
==ThT/DNA attachment==
 +
 
 +
1) Prepare the following concentrations of 4-strand and 8-strand telomeric DNA (which should generate 1 and 2 G-quadruplexes respectively)
 +
 
 +
a) 200nM
 +
 
 +
b) 400nM
 +
 
 +
c) 600nM
 +
 
 +
d) 800nM
 +
 
 +
e) 1uM
 +
 
 +
 

Revision as of 10:50, 25 July 2013

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To Do

  • Repeat Silica-Coating procedure using water to dilute nanoparticles instead of PBS. PBS is likely the cause of the aggregation we have been observing.[1]
  • Repeat ThT-DNA fluorescence procedure to show that fluorescence increases with increasing number of G-quadruplexes formed.

Silica-Coating Part 1

1) Dilute 23.5nM AuNPs to 9.4nM (2mL AuNPs in 3mL water *NOTE: the water was left to assimilate to room temperature prior to dilution*)

2)

ThT/DNA attachment

1) Prepare the following concentrations of 4-strand and 8-strand telomeric DNA (which should generate 1 and 2 G-quadruplexes respectively)

a) 200nM

b) 400nM

c) 600nM

d) 800nM

e) 1uM




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