User:Allison K. Alix/Notebook/Thesis Research/2013/07/25

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(ThT/DNA attachment)
(ThT/DNA attachment)
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==ThT/DNA attachment==
==ThT/DNA attachment==
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1) Prepare 200uL of the following concentrations of 4-strand and 8-strand telomeric DNA (which should generate 1 and 2 G-quadruplexes respectively)
+
1) Prepare 100uL of the following concentrations of 4-strand and 8-strand telomeric DNA (which should generate 1 and 2 G-quadruplexes respectively)
a) 100nM
a) 100nM
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d) 700nM
d) 700nM
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e) 900uM
+
e) 900nM
 +
 
 +
***Original Concentrations of 4 and 8-repeats are 265.6uM and 142.8uM, respectively***
 +
 
 +
4-repeat
 +
 
 +
1uM(1mL)=(265.6uM)x
 +
 
 +
x = 3.77uL in 996.23uL
 +
 
 +
900nM--> 90uL 1uM in 10uL PBS
 +
 
 +
700nM--> 70uL in 30uL
 +
 
 +
500nM--> 50uL in 50uL
 +
 
 +
300nM--> 30uL in 70uL
 +
 
 +
100nM--> 10uL in 90uL
 +
 
 +
 

Revision as of 11:09, 25 July 2013

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To Do

  • Repeat Silica-Coating procedure using water to dilute nanoparticles instead of PBS. PBS is likely the cause of the aggregation we have been observing.[1]
  • Repeat ThT-DNA fluorescence procedure to show that fluorescence increases with increasing number of G-quadruplexes formed.

Silica-Coating Part 1

1) Dilute 23.5nM AuNPs to 9.4nM (2mL AuNPs in 3mL water *NOTE: the water was left to assimilate to room temperature prior to dilution*)

2)

ThT/DNA attachment

1) Prepare 100uL of the following concentrations of 4-strand and 8-strand telomeric DNA (which should generate 1 and 2 G-quadruplexes respectively)

a) 100nM

b) 300nM

c) 500nM

d) 700nM

e) 900nM

      • Original Concentrations of 4 and 8-repeats are 265.6uM and 142.8uM, respectively***

4-repeat

1uM(1mL)=(265.6uM)x

x = 3.77uL in 996.23uL

900nM--> 90uL 1uM in 10uL PBS

700nM--> 70uL in 30uL

500nM--> 50uL in 50uL

300nM--> 30uL in 70uL

100nM--> 10uL in 90uL





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