User:Allison K. Alix/Notebook/Thesis Research/2013/07/25: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 14: Line 14:
==Silica-Coating Part 1==
==Silica-Coating Part 1==


1) Dilute 23.5nM AuNPs to 9.4nM (2mL AuNPs in 3mL water *NOTE: the water was left to assimilate to room temperature*)
1) Dilute 23.5nM AuNPs to 9.4nM (2mL AuNPs in 3mL water *NOTE: the water was left to assimilate to room temperature prior to dilution*)
 
2)
 
==ThT/DNA attachment==
 
1) Prepare the following concentrations of 4-strand and 8-strand telomeric DNA (which should generate 1 and 2 G-quadruplexes respectively)
 
a) 200nM
 
b) 400nM
 
c) 600nM
 
d) 800nM
 
e) 1uM
 
 




Revision as of 07:50, 25 July 2013

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

To Do

  • Repeat Silica-Coating procedure using water to dilute nanoparticles instead of PBS. PBS is likely the cause of the aggregation we have been observing.[1]
  • Repeat ThT-DNA fluorescence procedure to show that fluorescence increases with increasing number of G-quadruplexes formed.

Silica-Coating Part 1

1) Dilute 23.5nM AuNPs to 9.4nM (2mL AuNPs in 3mL water *NOTE: the water was left to assimilate to room temperature prior to dilution*)

2)

ThT/DNA attachment

1) Prepare the following concentrations of 4-strand and 8-strand telomeric DNA (which should generate 1 and 2 G-quadruplexes respectively)

a) 200nM

b) 400nM

c) 600nM

d) 800nM

e) 1uM




Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Allison_K._Alix/Notebook/Thesis_Research/2013/07/25&oldid=712134"