# User:Allison K. Alix/Notebook/Thesis Research/2013/07/25

(Difference between revisions)
 Revision as of 10:52, 25 July 2013 (view source) (→ThT/DNA attachment)← Previous diff Revision as of 11:09, 25 July 2013 (view source) (→ThT/DNA attachment)Next diff → Line 20: Line 20: ==ThT/DNA attachment== ==ThT/DNA attachment== - 1) Prepare 200uL of the following concentrations of 4-strand and 8-strand telomeric DNA (which should generate 1 and 2 G-quadruplexes respectively) + 1) Prepare 100uL of the following concentrations of 4-strand and 8-strand telomeric DNA (which should generate 1 and 2 G-quadruplexes respectively) a) 100nM a) 100nM Line 30: Line 30: d) 700nM d) 700nM - e) 900uM + e) 900nM + + ***Original Concentrations of 4 and 8-repeats are 265.6uM and 142.8uM, respectively*** + + 4-repeat + + 1uM(1mL)=(265.6uM)x + + x = 3.77uL in 996.23uL + + 900nM--> 90uL 1uM in 10uL PBS + + 700nM--> 70uL in 30uL + + 500nM--> 50uL in 50uL + + 300nM--> 30uL in 70uL + + 100nM--> 10uL in 90uL + +

## Revision as of 11:09, 25 July 2013

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## To Do

• Repeat Silica-Coating procedure using water to dilute nanoparticles instead of PBS. PBS is likely the cause of the aggregation we have been observing.[1]
• Repeat ThT-DNA fluorescence procedure to show that fluorescence increases with increasing number of G-quadruplexes formed.

## Silica-Coating Part 1

1) Dilute 23.5nM AuNPs to 9.4nM (2mL AuNPs in 3mL water *NOTE: the water was left to assimilate to room temperature prior to dilution*)

2)

## ThT/DNA attachment

1) Prepare 100uL of the following concentrations of 4-strand and 8-strand telomeric DNA (which should generate 1 and 2 G-quadruplexes respectively)

a) 100nM

b) 300nM

c) 500nM

d) 700nM

e) 900nM

• Original Concentrations of 4 and 8-repeats are 265.6uM and 142.8uM, respectively***

4-repeat

1uM(1mL)=(265.6uM)x

x = 3.77uL in 996.23uL

900nM--> 90uL 1uM in 10uL PBS

700nM--> 70uL in 30uL

500nM--> 50uL in 50uL

300nM--> 30uL in 70uL

100nM--> 10uL in 90uL