User:Allison K. Alix/Notebook/Thesis Research/2013/07/25
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To Do
Silica-Coating Part 11) Dilute 23.5nM AuNPs to 9.4nM (2mL AuNPs in 3mL water *NOTE: the water was left to assimilate to room temperature prior to dilution*) 2) ThT/DNA attachment1) Prepare 100uL of the following concentrations of 4-strand and 8-strand telomeric DNA (which should generate 1 and 2 G-quadruplexes respectively) a) 100nM b) 300nM c) 500nM d) 700nM e) 900nM
4-repeat x(500uL)=(265.6uM)(8.12) x = 4.31uM 1800nM--> 41.8uL in 58.2uL PBS 1400nM--> 32.5uL in 67.5uL 1000nM--> 23.2uL in 76.8uL 600nM--> 13.9uL in 86.1uL 200nM--> 4.6uL in 95.4uL 8-repeat 142.8uM(10uL) = (x)(500uL) x= 2.856 1800nM--> 63uL in 37uL PBS 1400nM--> 49uL in 51uL 1000nM--> 35uL in 65uL 600nM--> 21uL in 79uL 200nM--> 7uL in 93uL
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