User:Allison K. Alix/Notebook/Thesis Research/2013/08/13
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(→Scaled up SilicaCoating Procedure) 
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0.767x10^9moles/L x 1L/1000mL x 55.143mL = 4.23 x 10^11 moles AuNP total  0.767x10^9moles/L x 1L/1000mL x 55.143mL = 4.23 x 10^11 moles AuNP total  
  4.23 x 10^11 moles AuNP x 6.02 x 10^23 particles/mole = '''2.55 x 10^13 total Au nanoparticles'''  +  4.23 x 10^11 moles AuNP x 6.02 x 10^23 particles/mole = '''2.55 x 10^13 total Au nanoparticles''' 
+  
+  *We want a 5nM solution  
+  
+  5x10^9 moles/L x 1L/1000mL x 6.02x10^23 particles/mole = 3.01 x 10^12 particles/mL  
+  
+  2.55 x 10^13 particles / x = 3.01 x10^12 particles/1 mL  
+  
+  x = 8.47mL  
+  
+  Dilute AuNP in 8.47mL water after removing supernantant  
+  
+  8.47mL/24 vials = 353uL per vial.  
Revision as of 10:54, 13 August 2013
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Scaled up SilicaCoating ProcedureOriginal: 1mL AuNP & MHA solution/2μL TES/0.125mL NH4OH/5mL propanol 1) Rescale to use 9mL AuNP/MHA solution New: 9mL AuNP&MHA solution/18uL TES/1.125mL NH4OH/45mL propanol 2) Stir solutions for one hour to allow reaction to proceed 3) Centrifuge solutions at 4000 rpm for one hour 4) Resuspend particles in water CalculationsOriginal concentration of AuNP/MHA solution is 4.7nM 4.7nM(9mL)=55.143mL(x) x= 0.767nM 0.767x10^9moles/L x 1L/1000mL x 55.143mL = 4.23 x 10^11 moles AuNP total 4.23 x 10^11 moles AuNP x 6.02 x 10^23 particles/mole = 2.55 x 10^13 total Au nanoparticles
5x10^9 moles/L x 1L/1000mL x 6.02x10^23 particles/mole = 3.01 x 10^12 particles/mL 2.55 x 10^13 particles / x = 3.01 x10^12 particles/1 mL x = 8.47mL Dilute AuNP in 8.47mL water after removing supernantant 8.47mL/24 vials = 353uL per vial.
