# User:Allison K. Alix/Notebook/Thesis Research/2013/08/13

(Difference between revisions)
 Revision as of 11:22, 13 August 2013 (view source) (→Entry title)← Previous diff Revision as of 11:34, 13 August 2013 (view source) (→Scaled up Silica-Coating Procedure)Next diff → Line 19: Line 19: 4) Re-suspend particles in water 4) Re-suspend particles in water + + ==Calculations== + + Original concentration of AuNP/MHA solution is 4.7nM + + 4.7nM(9mL)=55.143mL(x) + + x= 0.767nM + + 0.767x10^-9moles/L x 1L/1000mL x 55.143mL = 4.23 x 10^-11 moles AuNP total + + 4.23 x 10^-11 moles AuNP x 6.02 x 10^23 particles/mole = '''2.55 x 10^13 total Au nanoparticles'''

## Revision as of 11:34, 13 August 2013

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## Scaled up Silica-Coating Procedure

Original: 1mL AuNP & MHA solution/2μL TES/0.125mL NH4OH/5mL propanol

1) Re-scale to use 9mL AuNP/MHA solution

New: 9mL AuNP&MHA solution/18uL TES/1.125mL NH4OH/45mL propanol

2) Stir solutions for one hour to allow reaction to proceed

3) Centrifuge solutions at 4000 rpm for one hour

4) Re-suspend particles in water

## Calculations

Original concentration of AuNP/MHA solution is 4.7nM

4.7nM(9mL)=55.143mL(x)

x= 0.767nM

0.767x10^-9moles/L x 1L/1000mL x 55.143mL = 4.23 x 10^-11 moles AuNP total

4.23 x 10^-11 moles AuNP x 6.02 x 10^23 particles/mole = 2.55 x 10^13 total Au nanoparticles