# User:Allison K. Alix/Notebook/Thesis Research/2013/08/13

(Difference between revisions)
 Revision as of 10:58, 13 August 2013 (view source) (→Scaled up Silica-Coating Procedure)← Previous diff Revision as of 11:58, 13 August 2013 (view source) (→DNA Attachment)Next diff → Line 45: Line 45: ==DNA Attachment== ==DNA Attachment== + + 1) Mix 300uL AuNP@SiO2 with 30uL APS and 470uL 100% ethanol. Sonicate for 30 minutes

## Revision as of 11:58, 13 August 2013

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## Scaled up Silica-Coating Procedure

Original: 1mL AuNP & MHA solution/2μL TES/0.125mL NH4OH/5mL 2-propanol

1) Re-scale to use 9mL AuNP/MHA solution

New: 9mL AuNP&MHA solution/18uL TES/1.125mL NH4OH/45mL 2-propanol

2) Stir solutions for one hour to allow reaction to proceed

3) Centrifuge solutions at 4000 rpm for one hour

4) Re-suspend particles in water

## Calculations

Original concentration of AuNP/MHA solution is 4.7nM

4.7nM(9mL)=55.143mL(x)

x= 0.767nM

0.767x10^-9moles/L x 1L/1000mL x 55.143mL = 4.23 x 10^-11 moles AuNP total

4.23 x 10^-11 moles AuNP x 6.02 x 10^23 particles/mole = 2.55 x 10^13 total Au nanoparticles

• We want a 5nM solution

5x10^-9 moles/L x 1L/1000mL x 6.02x10^23 particles/mole = 3.01 x 10^12 particles/mL

2.55 x 10^13 particles / x = 3.01 x10^12 particles/1 mL

x = 8.47mL

Dilute AuNP in 8.47mL water after removing supernantant

8.47mL/24 vials = 353uL per vial.

## DNA Attachment

1) Mix 300uL AuNP@SiO2 with 30uL APS and 470uL 100% ethanol. Sonicate for 30 minutes