User:Allison K. Alix/Notebook/Thesis Research/2013/08/27: Difference between revisions
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[ThT] must be twice what [DNA] is | [ThT] must be twice what [DNA] is | ||
[DNA] = | [DNA] = 0.624μM, so [ThT] must be 1.248μM | ||
Use | Use 150μL of DNA-AuNP solution (10%=15μL) | ||
1.248x10-6M x 165x10-6L solution = 2.0592x10-10 moles ThT | |||
( | (2.0592x10-10 moles ThT)/ 15 x 10-6 L = 13.728μM | ||
-Add | -Add 15μL of 13.728μM to 150μL thiol-DNA/AuNP solution | ||
NEW Concentrations: | NEW Concentrations: | ||
[DNA]= | [DNA]= 0.567μM, [ThT] = 1.248μM | ||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | ||
Revision as of 08:46, 27 August 2013
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To Do
Au@SiO2/DNA UV-Vis Data
ε of thiol-DNA= 607500 A=0.379 b=1 cm A=εbc c=A/εb c = 6.23x10^-7M = 0.624uM Addition of ThT[ThT] must be twice what [DNA] is [DNA] = 0.624μM, so [ThT] must be 1.248μM Use 150μL of DNA-AuNP solution (10%=15μL) 1.248x10-6M x 165x10-6L solution = 2.0592x10-10 moles ThT (2.0592x10-10 moles ThT)/ 15 x 10-6 L = 13.728μM -Add 15μL of 13.728μM to 150μL thiol-DNA/AuNP solution NEW Concentrations: [DNA]= 0.567μM, [ThT] = 1.248μM | |
