User:Allison K. Alix/Notebook/Thesis Research/2013/08/27: Difference between revisions
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c = 6.23x10^-7M = 0.624uM | c = 6.23x10^-7M = 0.624uM | ||
== | ==Addition of ThT== | ||
[ThT] must be twice what [DNA] is | |||
2 | [DNA] = 1.11225μM, so [ThT] must be 2.2245μM | ||
Use 250μL of DNA-AuNP solution (10%=25μM) | |||
2.2245x10-6M x 275x10-6L solution = 6.117375x10-10 moles ThT | |||
(6.117375x10-10 moles ThT)/ 25 x 10-6 L = 24.4695μM | |||
-Add 25μL of 24.4695μM ThT to 250μL thiol-DNA/AuNP solution | |||
NEW Concentrations: | |||
[DNA]= 1.011μM [AuNP] = 13.486μM [ThT] = 2.2245μM | |||
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Revision as of 08:41, 27 August 2013
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To Do
Au@SiO2/DNA UV-Vis Data
ε of thiol-DNA= 607500 A=0.379 b=1 cm A=εbc c=A/εb c = 6.23x10^-7M = 0.624uM Addition of ThT[ThT] must be twice what [DNA] is [DNA] = 1.11225μM, so [ThT] must be 2.2245μM Use 250μL of DNA-AuNP solution (10%=25μM) 2.2245x10-6M x 275x10-6L solution = 6.117375x10-10 moles ThT (6.117375x10-10 moles ThT)/ 25 x 10-6 L = 24.4695μM -Add 25μL of 24.4695μM ThT to 250μL thiol-DNA/AuNP solution NEW Concentrations: [DNA]= 1.011μM [AuNP] = 13.486μM [ThT] = 2.2245μM | |
