User:Allison K. Alix/Notebook/Thesis Research/2013/09/04: Difference between revisions
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==Fluorescence Measurements== | ==Fluorescence Measurements== | ||
[[Image:Au@SiO2_DNA_ThT.png]] | |||
Revision as of 08:30, 4 September 2013
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Absorbance Measurements of Au@SiO@/DNA (8/29/13)
ε of thiol-DNA= 607500 A=0.553 b=1 cm A=εbc c=A/εb c = 9.10x10^-7M = 0.910uM Addition of ThT[ThT] must be twice what [DNA] is [DNA] = 0.910μM, so [ThT] must be 1.82μM Use 150μL of DNA-AuNP solution (10%=15μL) 1.820x10-6M x 165x10-6L solution = 3.003x10-10 moles ThT (3.003x10-10 moles ThT)/ 15 x 10-6 L = 20.02μM -Add 15μL of 20.2μM to 150μL thiol-DNA/AuNP solution NEW Concentrations: [DNA]= 0.827μM, [ThT] = 1.82μM 1) Prepare 100uL 20uM ThT (use already prepared 5/14/13) 2) React with DNA ten minutes 3) Observe absorbance and fluorescence Absorbance Measurements of Au@SiO2/ThT-DNAFluorescence Measurements
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