User:Allison K. Alix/Notebook/Thesis Research/2013/09/10: Difference between revisions
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4) Observe absorbance and fluorescence (absorbance peak should be ~532nm) | 4) Observe absorbance and fluorescence (absorbance peak should be ~532nm) | ||
==Fluorescence Spectrum of MB== | |||
[Insert Fluor spec] | |||
The Au@SiO@/DNA-MB complex showed an intensity of 80 (relative intensity 0.08) when excited at 532 nm. | |||
==FCS Measurements== | |||
FCS Measurements were taken of 10X dilute, 50X diluted, and 100X diluted Au@SiO2/DNA-MB samples. No correlation curve was present. | |||
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Revision as of 14:01, 11 September 2013
 Project name
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To Do
Addition of MB[DNA] = 0.910μM, so [MB] must be at least in 1:1 ratio [MB] original = 7.61uM 1) Add 20uL 7.61uM MB to 150uL Au/SiO2/DNA solution [MB] = 0.895uM [DNA] = 0.802
2) Heat sample for ~20 minutes to stimulate hybridization 3) Cool to room temperature on hot plate 4) Observe absorbance and fluorescence (absorbance peak should be ~532nm) Fluorescence Spectrum of MB[Insert Fluor spec] The Au@SiO@/DNA-MB complex showed an intensity of 80 (relative intensity 0.08) when excited at 532 nm. FCS MeasurementsFCS Measurements were taken of 10X dilute, 50X diluted, and 100X diluted Au@SiO2/DNA-MB samples. No correlation curve was present. | |
