User:Allison K. Alix/Notebook/Thesis Research/2013/09/12

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(FCS Measurements of Antibody-Alexa 532 Pt 2)
(FCS Measurements of Antibody-Alexa 532 Pt 2)
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| 10nM || 3.50x10<sup>3</sup>||  
| 10nM || 3.50x10<sup>3</sup>||  
|-
|-
-
| 1nM || 2.00x10<sup>3</sup> ||  
+
| 1nM || 2.30x10<sup>3</sup> ||
 +
|-
 +
| 100pM || 1.98x10<sup>3</sup>||
 +
|-
 +
| 10pM || 2.30x10<sup>3</sup> ||  
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Revision as of 13:00, 12 September 2013

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FCS Measurements of Antibody-Alexa 532 Pt 2

  • Green fluorescent beads were first used to calibrate the FCS (See spectra below)
  • The count rate of each Antibody/Alexa 532 sample was monitored
Sample Concentration TDC counts/sec
100nM 1.90x104
10nM 3.50x103
1nM 2.30x103
100pM 1.98x103
10pM 2.30x103

FCS Spectra

Image:1000X_diluted_green_beads_pic.png



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