# User:Allison K. Alix/Notebook/Thesis Research/2013/09/24

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 Revision as of 09:19, 24 September 2013 (view source) (→Calculating [AuNP] in Au@SiO2/DNA-MB solutions)← Previous diff Revision as of 09:27, 24 September 2013 (view source) (→Calculating [AuNP] in Au@SiO2/DNA-MB solutions)Next diff → Line 33: Line 33: x = 6.25nM x = 6.25nM + + 5) Addition of 13.3uL 7.61uM MB to 100uL Au@SiO2/DNA + + 6.25nM(100uL)=x(113.3uL) + + x = 5.52nM

## Revision as of 09:27, 24 September 2013

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## Calculating [AuNP] in Au@SiO2/DNA-MB solutions

• Original [Au@SiO2] = 5nM before attaching DNA

1) Addition of 5uL APS

(1mL)(5nM)=(1.005mL)(x)

x = 4.975nM

2) Addition of 1.5mL Sulfo-SMCC

(1.005mL)(4.975nM) = (x)(2.505mL)

x = 1.996nM

3) Centrifugation, removal of supernatant, and redispersion in 530uL KP buffer

(1.996nM)(2.505mL) = x(0.530mL)

x = 9.43nM

4) Addition of 270uL DNA

(9.43nM)(530uL)=x(800uL)

x = 6.25nM

5) Addition of 13.3uL 7.61uM MB to 100uL Au@SiO2/DNA

6.25nM(100uL)=x(113.3uL)

x = 5.52nM