User:Allison K. Alix/Notebook/Thesis Research/2013/09/24

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(Calculating [AuNP] in Au@SiO2/DNA-MB solutions)
(Calculating [AuNP] in Au@SiO2/DNA-MB solutions)
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x = 5.52nM
x = 5.52nM
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{| class="wikitable"
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|-
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! Dilution Factor !! [Au@SiO<sub>2</sub>] !! [DNA] !! [MB]
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|-
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| None (stock) || 5.52nM || 802nM || 895nM
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|-
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| 2X || 2.76nM || 401nM || 447.5nM
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|-
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| 10X || 552pM || 80.2nM || 89.5nM
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|-
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| 100X || 55.2pM || 8.02nM || 8.95nM
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|-
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| 1000X || 5.52pM || 802pM || 895pM
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|-
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| 10000X || 552fM || 80.2pM || 89.5pM
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|}

Revision as of 10:39, 24 September 2013

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Calculating [AuNP] in Au@SiO2/DNA-MB solutions

  • Original [Au@SiO2] = 5nM before attaching DNA

1) Addition of 5uL APS

(1mL)(5nM)=(1.005mL)(x)

x = 4.975nM

2) Addition of 1.5mL Sulfo-SMCC

(1.005mL)(4.975nM) = (x)(2.505mL)

x = 1.996nM

3) Centrifugation, removal of supernatant, and redispersion in 530uL KP buffer

(1.996nM)(2.505mL) = x(0.530mL)

x = 9.43nM

4) Addition of 270uL DNA

(9.43nM)(530uL)=x(800uL)

x = 6.25nM

5) Addition of 13.3uL 7.61uM MB to 100uL Au@SiO2/DNA

6.25nM(100uL)=x(113.3uL)

x = 5.52nM


Dilution Factor [Au@SiO2] [DNA] [MB]
None (stock) 5.52nM 802nM 895nM
2X 2.76nM 401nM 447.5nM
10X 552pM 80.2nM 89.5nM
100X 55.2pM 8.02nM 8.95nM
1000X 5.52pM 802pM 895pM
10000X 552fM 80.2pM 89.5pM






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