User:Allison K. Alix/Notebook/Thesis Research/2013/10/09: Difference between revisions
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==Calculations for preparing diltuions== | |||
* Need 20uL for each dilution | |||
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! Dilution Factor !! Solution to make dilution from !! Volume of solution !! Volume of PBS | |||
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| 5X || stock || 4uL || 16uL | |||
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| 10X || stock || 3uL || 27uL | |||
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| 100X || 10X || 3uL || 27uL | |||
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| 1000X || 100X || 3uL || 27uL | |||
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| 10000X || 1000X || 3uL || 27uL | |||
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Revision as of 09:33, 9 October 2013
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To Do
Preparing new Au@SiO2-DNA/MB solutions
1) 1:3 DNA:MB Original [DNA] = 910nM Original [MB] = 7610nM MB should be 2730nM
NEW CONCENTRATIONS: MB = 3.182uM DNA = 404uM (This is actually a 1:8 ratio....) 2) 1:1 Added 3.3uL 7.61uM MB to 25uL Au@Si/DNA solution [MB] = 887nM [DNA] = 803nM 3) 3:1 DNA:MB Added 1uL MB to 25uL Au@Si/DNA solution [MB] = 292.7nM [DNA] = 875nM Dilutions for DNA:MB samples1)
2)
3)
Calculations for preparing diltuions
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