User:Allison K. Alix/Notebook/Thesis Research/2013/10/29: Difference between revisions
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* The second thing to check is that the known concentration of MB is actually what was calculated. Although these samples were made straight from a stock solution with a known concentration, there could have been error during preparation, that is affecting the actual concentration that is not being accounted for. To check the concentrations, new MB samples will be prepared, and their UV-Vis spectra will be obtained | * The second thing to check is that the known concentration of MB is actually what was calculated. Although these samples were made straight from a stock solution with a known concentration, there could have been error during preparation, that is affecting the actual concentration that is not being accounted for. To check the concentrations, new MB samples will be prepared, and their UV-Vis spectra will be obtained | ||
==Data== | |||
Revision as of 08:37, 30 October 2013
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To Do
Calculating %MB openExample: N = 7.9 particles 7.9 particles x 1 mol/6.02x1023 particles x 1/6.93 x 10-16L = 1.89x10-8M NOTE: 6.93x10-16 is the focal volume of the spot, previous calculated 1.89x10-8M x 1nM/10-9M = 18.9nM Actual concentration of this sample was 22.4nM (According to dilution calculations) %MB open = 18.9nM/22.4nM x 100% = 84.4nM
Data |