User:Allison K. Alix/Notebook/Thesis Research/2013/10/29: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> October 29th 2013</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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==Data== | ==Data== | ||
[[Image:Green_bead_igor_curve.png]] | |||
The above image is an FCS curve showing the average diffusion time of 9 10000X diluted green bead samples. τ<sub>D</sub> was calculated to be 58.7ms | |||
==Calculations== | |||
'''radius of the spot''' | |||
τ<sub>D</sub> = (ω<sub>0</sub><sup>2</sup>3ηπd)/4kT | |||
where τ<sub>D</sub> is the diffusion time of the sample | |||
ω<sub>0</sub> is the radius of the spot | |||
η is the viscosity of the solvent (water), 0.001028kg/m s | |||
d is the diameter of the particle | |||
k is Boltzmann's constant = 1.3806488 × 10-23 m<sup>2</sup> kg s<sup>-2</sup>-2 K<sup>-1</sup> | |||
T is the temperature of the room (18.7 °C, 291.7K) | |||
ω<sub>0</sub> = sqrt([(0.05874s(4)(1.3806488x10<sup>-23</sup>m<sup>2</sup>kg/s<sup>2</sup>K)(291.7K)]/[3(0.001028kg/m s)(π)(2x10<sup>-7</sup>m)]) | |||
ω<sub>0</sub> = 0.698μm | |||
'''volume of the spot''' | |||
V =4/3(π)r<sup>3</sup> | |||
= 4/3(π)(6.98x10<sup>-7</sup>)<sup>3</sup> | |||
= 1.42x10<sup>-18</sup> x 1000L/1m<sup>3</sup> | |||
= 1.42x10<sup>-15</sup>L | |||
{| class="wikitable" | |||
|- | |||
! [MB](nM) !! %MB open !! | |||
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| 22.4 || 39.3 | |||
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| 17.9 || 26.2 | |||
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| 14.9 || 30.6 | |||
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| 12.8 || 34.1 | |||
|- | |||
| 11.2 || 50.7 | |||
|- | |||
| 9.9 || 40.1 | |||
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| 8.9 || 48.2 | |||
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| 8.2 || 62.3 | |||
|} | |||
Revision as of 13:09, 5 November 2013
October 29th 2013 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||
To Do
Calculating %MB openExample: N = 7.9 particles 7.9 particles x 1 mol/6.02x1023 particles x 1/6.93 x 10-16L = 1.89x10-8M NOTE: 6.93x10-16 is the focal volume of the spot, previous calculated 1.89x10-8M x 1nM/10-9M = 18.9nM Actual concentration of this sample was 22.4nM (According to dilution calculations) %MB open = 18.9nM/22.4nM x 100% = 84.4nM
DataThe above image is an FCS curve showing the average diffusion time of 9 10000X diluted green bead samples. τD was calculated to be 58.7ms Calculationsradius of the spot τD = (ω023ηπd)/4kT where τD is the diffusion time of the sample ω0 is the radius of the spot η is the viscosity of the solvent (water), 0.001028kg/m s d is the diameter of the particle k is Boltzmann's constant = 1.3806488 × 10-23 m2 kg s-2-2 K-1 T is the temperature of the room (18.7 °C, 291.7K) ω0 = sqrt([(0.05874s(4)(1.3806488x10-23m2kg/s2K)(291.7K)]/[3(0.001028kg/m s)(π)(2x10-7m)]) ω0 = 0.698μm volume of the spot V =4/3(π)r3 = 4/3(π)(6.98x10-7)3 = 1.42x10-18 x 1000L/1m3 = 1.42x10-15L
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