User:Allison K. Alix/Notebook/Thesis Research/2013/10/30

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(Sample Preparation)
(To Do)
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* Prepare MB samples and obtain UV-Vis Spectra. Calculate the concentration of each.
* Prepare MB samples and obtain UV-Vis Spectra. Calculate the concentration of each.
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* Attach DNA to AuNP (no silica coating) obtain concentration of DNA attached to AuNP, attach MB.
==Sample Preparation==
==Sample Preparation==

Revision as of 13:00, 30 October 2013

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To Do

  • Prepare MB samples and obtain UV-Vis Spectra. Calculate the concentration of each.
  • Attach DNA to AuNP (no silica coating) obtain concentration of DNA attached to AuNP, attach MB.

Sample Preparation

Prepare 150uL of the following MB samples with the following concentrations:

22.4nM, 17.9nM, 14.9nM, 12.8nM, 11.2nM, 9.9nM, 8.9nM, and 8.2nM

Original MB concentration: 7.61uM

Dilute to 895nM

7.61x10-6M (x) = 895x10-9M(75uL)

x = 8.8uL in 66.2uL PBS



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