User:Allison K. Alix/Notebook/Thesis Research/2013/11/05: Difference between revisions
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2) Heat for 25 min at ~76.0°C | 2) Heat for 25 min at ~76.0°C | ||
3 | 3) Run FCS Measurements. Begin with pure sample. Because the AuNP concentration is so low (290pM) it might be hard to get signal from anything lower. | ||
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Revision as of 12:53, 5 November 2013
 Project name
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To Do
Data
Calculating AuNP/DNA ConcentrationAuNP: abs @ 532 = 0.03 ε = 92,382,878 c= A/εb c = 3.2473x10-10M = 0.325nM DNA: abs @ 254 = 0.399 ε = 607,500 c = 6.5679E-07M = 656.6nM Addition of MB1) Add 6uL 7.61uM MB to 50uL AuNP/DNA Solution NEW Concentrations: MB: 815nM DNA: 586nM AuNP: 0.29nM 2) Heat for 25 min at ~76.0°C 3) Run FCS Measurements. Begin with pure sample. Because the AuNP concentration is so low (290pM) it might be hard to get signal from anything lower. | |
