User:Allison K. Alix/Notebook/Thesis Research/2013/11/05

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(Data)
(Data)
Line 16: Line 16:
[[Image:OWW_AUNP_DNA_pic.png]]
[[Image:OWW_AUNP_DNA_pic.png]]
-
Calculating AuNP/DNA Concentration==
+
==Calculating AuNP/DNA Concentration==
AuNP: abs @ 532 = 0.03
AuNP: abs @ 532 = 0.03
 +
ε = 92,382,878
ε = 92,382,878
c= A/εb
c= A/εb
 +
c = 3.2473x10<sup>-10</sup>M = 0.325nM
c = 3.2473x10<sup>-10</sup>M = 0.325nM
DNA: abs @ 254 = 0.399
DNA: abs @ 254 = 0.399
 +
ε = 607,500
ε = 607,500
 +
c = 6.5679E-07M = 656.6nM
c = 6.5679E-07M = 656.6nM

Revision as of 13:49, 5 November 2013

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To Do

  • Run UV-Vis of MB samples to determine concentraiton
  • Add MB to AuNP/DNA Sample. Run FCS.

Data

Image:OWW_AUNP_DNA_pic.png

Calculating AuNP/DNA Concentration

AuNP: abs @ 532 = 0.03

ε = 92,382,878

c= A/εb

c = 3.2473x10-10M = 0.325nM

DNA: abs @ 254 = 0.399

ε = 607,500

c = 6.5679E-07M = 656.6nM



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