# User:Allison K. Alix/Notebook/Thesis Research/2013/11/05

(Difference between revisions)
 Revision as of 13:49, 5 November 2013 (view source) (→Data)← Previous diff Revision as of 13:49, 5 November 2013 (view source) (→Data)Next diff → Line 16: Line 16: [[Image:OWW_AUNP_DNA_pic.png]] [[Image:OWW_AUNP_DNA_pic.png]] - Calculating AuNP/DNA Concentration== + ==Calculating AuNP/DNA Concentration== AuNP: abs @ 532 = 0.03 AuNP: abs @ 532 = 0.03 + ε = 92,382,878 ε = 92,382,878 c= A/εb c= A/εb + c = 3.2473x10-10M = 0.325nM c = 3.2473x10-10M = 0.325nM DNA: abs @ 254 = 0.399 DNA: abs @ 254 = 0.399 + ε = 607,500 ε = 607,500 + c = 6.5679E-07M = 656.6nM c = 6.5679E-07M = 656.6nM

## Revision as of 13:49, 5 November 2013

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## To Do

• Run UV-Vis of MB samples to determine concentraiton
• Add MB to AuNP/DNA Sample. Run FCS.

## Calculating AuNP/DNA Concentration

AuNP: abs @ 532 = 0.03

ε = 92,382,878

c= A/εb

c = 3.2473x10-10M = 0.325nM

DNA: abs @ 254 = 0.399

ε = 607,500

c = 6.5679E-07M = 656.6nM