User:Aman Kumar: Difference between revisions
Aman Kumar (talk | contribs) |
Aman Kumar (talk | contribs) |
||
| Line 33: | Line 33: | ||
<br> | <br> | ||
<p><b>Abstract: </b>Shiga toxin, a worldwide menace, has killed over 1 million people to date and continues to afflict almost 150 million people each year. Currently, there is no treatment for Shiga toxicosis and it leads to complications in the human system like hemolytic uremic syndrome (HUS) and renal failure. Here, we propose a two-fold, novel synthetic biology approach to combat the lethal effect of the toxin. We aim to neutralize the already produced toxin through a nine amino acid Gb3 mimic peptide. We have engineered the Gb3 mimic along with a cellular export signal (ompF) downstream of AHL(quorum sensing molecule) inducible promoter (pLuxR). We also plan to prevent further toxin production by inhibiting the biofilm formation of shigatoxigenic E.coli using indole-3-acetaldehyde (I3A). We expect to validate our approach through functional assays and in silico modelling. Our findings can potentially initiate a new perspective of tackling Shiga toxicosis using synthetic biology tools. | <p><b>Abstract: </b>Shiga toxin, a worldwide menace, has killed over 1 million people to date and continues to afflict almost 150 million people each year. Currently, there is no treatment for Shiga toxicosis and it leads to complications in the human system like hemolytic uremic syndrome (HUS) and renal failure. Here, we propose a two-fold, novel synthetic biology approach to combat the lethal effect of the toxin. We aim to neutralize the already produced toxin through a nine amino acid Gb3 mimic peptide. We have engineered the Gb3 mimic along with a cellular export signal (ompF) downstream of AHL(quorum sensing molecule) inducible promoter (pLuxR). We also plan to prevent further toxin production by inhibiting the biofilm formation of shigatoxigenic E.coli using indole-3-acetaldehyde (I3A). We expect to validate our approach through functional assays and in silico modelling. Our findings can potentially initiate a new perspective of tackling Shiga toxicosis using synthetic biology tools. | ||
Please visit our <a href="http://2013.igem.org/Team:IIT_Madras"> official iGEM website </a> for more information. | |||
==Publications== | ==Publications== | ||
Revision as of 10:03, 21 November 2013
Contact Info
<html><b>Aman Kumar</b></html>
<html><br></html>
258 Alakananda Hostel, Indian Institute of Technology (IIT) Madras, Chennai, Tamil Nadu, India.
<html><br>Email:<a href="mailto:amank.iitm@gmail.com">amank.iitm@gmail.com</a></html>
<html><br>Email:<a href="mailto:bt10b003@smail.iitm.ac.in">bt10b003@smail.iitm.ac.in</a><br></html>
Education
<html> <ul> <li><b>Master of technology in Biotechnology, <a href="http://www.iitm.ac.in/">IIT Madras </a> (2015)<br> Bachelor of technology in Biotechnology, <a href="http://www.iitm.ac.in/">IIT Madras </a> (2014)</b></li> <li><b>Board of Higher Secondary Education (2008)<br> School: Patna Central School</b></li> Graduated with 91% (Topper of the class and 7th rank holder in the school of 1200 students)<br> <li><b>Board of Secondary Education (2006)<br> School: Patna Central School </b></li> Graduated with 87.2% </ul> </html>
Research Projects
<html>
- International Genetically Engineered Machine (iGEM) Progect 2013: Combating Shiga-toxin: A synthetic biology approach [<a href="http://www.biotech.iitm.ac.in/faculty/nitish.htm"> Dr. Nitish Mahapatra </a>,<a href="https://home.iitm.ac.in/kraman/lab/"> Dr. Karthik Raman </a>,; Mar 2013 - Sept 2013]
- Goldbeter A and Koshland DE Jr. An amplified sensitivity arising from covalent modification in biological systems. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6840-4. DOI:10.1073/pnas.78.11.6840 |
- JACOB F and MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318-56. DOI:10.1016/s0022-2836(61)80072-7 |
leave a comment about a paper here
- ISBN:0879697164
Abstract: Shiga toxin, a worldwide menace, has killed over 1 million people to date and continues to afflict almost 150 million people each year. Currently, there is no treatment for Shiga toxicosis and it leads to complications in the human system like hemolytic uremic syndrome (HUS) and renal failure. Here, we propose a two-fold, novel synthetic biology approach to combat the lethal effect of the toxin. We aim to neutralize the already produced toxin through a nine amino acid Gb3 mimic peptide. We have engineered the Gb3 mimic along with a cellular export signal (ompF) downstream of AHL(quorum sensing molecule) inducible promoter (pLuxR). We also plan to prevent further toxin production by inhibiting the biofilm formation of shigatoxigenic E.coli using indole-3-acetaldehyde (I3A). We expect to validate our approach through functional assays and in silico modelling. Our findings can potentially initiate a new perspective of tackling Shiga toxicosis using synthetic biology tools. Please visit our <a href="http://2013.igem.org/Team:IIT_Madras"> official iGEM website </a> for more information.
