User:Aman Kumar

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(Research Projects)
(Research Projects)
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<li><b>Characterization of Chromogranin A protein and studying its interaction with HSPs.</b></li>
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<li><b>Characterization of Chromogranin A protein and studying its interaction with Hsp.</b></li>
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[<a href="http://www.biotech.iitm.ac.in/faculty/nitish.htm"> Dr. Nitish Mahapatra </a>,; Dec 2012 - Till Date]
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<p><b>Abstract: </b>Cardiovascular diseases (coronary heart disease, heart failure, stroke etc.) affect a large section of the adult population and are the leading causes of morbidity and mortality in many countries including India. Chromogranin A (CHGA) is ubiquitously expressed in secretory cells of the endocrine, neuroendocrine, and neuronal tissues. Although this protein has long been known as a marker for neuroendocrine tumors, its role in cardiovascular disease states including essential hypertension (EH) has only recently been recognized. Our plan focuses on purifying and investigating the functional behaviour of the protein and study its interactions with heat shock proteins (Hsp 70).

Revision as of 13:37, 21 November 2013

Contact Info

Aman Kumar representing iGEM'13 IIT Madras team at CUHK, Hong Kong
Aman Kumar representing iGEM'13 IIT Madras team at CUHK, Hong Kong


Aman Kumar
258 Alakananda Hostel, Indian Institute of Technology (IIT) Madras, Chennai, Tamil Nadu, India.
Email:amank.iitm@gmail.com
Email:bt10b003@smail.iitm.ac.in


Education

  • Master of technology in Biotechnology, IIT Madras (2015)
    Bachelor of technology in Biotechnology, IIT Madras (2014)
  • Board of Higher Secondary Education (2008)
    School: Patna Central School
  • Graduated with 91% (Topper of the class and 7th rank holder in the school of 1200 students)
  • Board of Secondary Education (2006)
    School: Patna Central School
  • Graduated with 87.2%

Research Projects

  • International Genetically Engineered Machine (iGEM) Progect 2013: Combating Shiga-toxin: A synthetic biology approach
  • [ Dr. Nitish Mahapatra , Dr. Karthik Raman ,; Mar 2013 - Sept 2013]

    Abstract: Shiga toxin, a worldwide menace, has killed over 1 million people to date and continues to afflict almost 150 million people each year. Currently, there is no treatment for Shiga toxicosis and it leads to complications in the human system like hemolytic uremic syndrome (HUS) and renal failure. Here, we propose a two-fold, novel synthetic biology approach to combat the lethal effect of the toxin. We aim to neutralize the already produced toxin through a nine amino acid Gb3 mimic peptide. We have engineered the Gb3 mimic along with a cellular export signal (ompF) downstream of AHL(quorum sensing molecule) inducible promoter (pLuxR). We also plan to prevent further toxin production by inhibiting the biofilm formation of shigatoxigenic E.coli using indole-3-acetaldehyde (I3A). We expect to validate our approach through functional assays and in silico modelling. Our findings can potentially initiate a new perspective of tackling Shiga toxicosis using synthetic biology tools.
    Please visit our official iGEM'13 website for more information.

  • Characterization of Chromogranin A protein and studying its interaction with Hsp.
  • [ Dr. Nitish Mahapatra ,; Dec 2012 - Till Date]

    Abstract: Cardiovascular diseases (coronary heart disease, heart failure, stroke etc.) affect a large section of the adult population and are the leading causes of morbidity and mortality in many countries including India. Chromogranin A (CHGA) is ubiquitously expressed in secretory cells of the endocrine, neuroendocrine, and neuronal tissues. Although this protein has long been known as a marker for neuroendocrine tumors, its role in cardiovascular disease states including essential hypertension (EH) has only recently been recognized. Our plan focuses on purifying and investigating the functional behaviour of the protein and study its interactions with heat shock proteins (Hsp 70).

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