User:Aman Kumar

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Aman Kumar representing iGEM'13 IIT Madras team at CUHK, Hong Kong
Aman Kumar representing iGEM'13 IIT Madras team at CUHK, Hong Kong


Aman Kumar
258 Alakananda Hostel, Indian Institute of Technology (IIT) Madras, Chennai, Tamil Nadu, India.
Email:amank.iitm@gmail.com
Email:bt10b003@smail.iitm.ac.in


Education

  • Master of technology in Biotechnology, IIT Madras (2015)
    Bachelor of technology in Biotechnology, IIT Madras (2014)
  • Board of Higher Secondary Education (2009)
    School: Patna Central School
  • Graduated with 91% (Class ranking = 1st (among 100 students)) (School ranking = 7th (among 1200 students))
  • Board of Secondary Education (2007)
    School: Patna Central School
  • Graduated with 87.2%

Research Projects

  • International Genetically Engineered Machine (iGEM) Progect 2013: Combating Shiga-toxin: A synthetic biology approach
  • [ Dr. Nitish Mahapatra , Dr. Karthik Raman ,; Mar 2013 - Sept 2013]

    Abstract: Shiga toxin, a worldwide menace, has killed over 1 million people to date and continues to afflict almost 150 million people each year. Currently, there is no treatment for Shiga toxicosis and it leads to complications in the human system like hemolytic uremic syndrome (HUS) and renal failure. Here, we propose a two-fold, novel synthetic biology approach to combat the lethal effect of the toxin. We aim to neutralize the already produced toxin through a nine amino acid Gb3 mimic peptide. We have engineered the Gb3 mimic along with a cellular export signal (ompF) downstream of AHL(quorum sensing molecule) inducible promoter (pLuxR). We also plan to prevent further toxin production by inhibiting the biofilm formation of shigatoxigenic E.coli using indole-3-acetaldehyde (I3A). We expect to validate our approach through functional assays and in silico modelling. Our findings can potentially initiate a new perspective of tackling Shiga toxicosis using synthetic biology tools.
    Please visit our official iGEM'13 website for more information.

  • Characterization of Chromogranin A protein and studying its interaction with Hsp.
  • [ Dr. Nitish Mahapatra ,; Dec 2012 - Till Date]

    Abstract: Cardiovascular diseases (coronary heart disease, heart failure, stroke etc.) affect a large section of the adult population and are the leading causes of morbidity and mortality in many countries including India. Chromogranin A (CHGA) is ubiquitously expressed in secretory cells of the endocrine, neuroendocrine, and neuronal tissues. Although this protein has long been known as a marker for neuroendocrine tumors, its role in cardiovascular disease states including essential hypertension (EH) has only recently been recognized. Our plan focuses on purifying and investigating the functional behaviour of the protein and study its interactions with heat shock proteins (Hsp 70).

  • Summer Internship: Investigating the function of Mom protein using site-directed mutagenesis.
  • [ Prof. V Nagaraja,; May'12 - Jun'12]

    Abstract: Bacteriophage Mu or phage Mu is a temperate bacteriophage that infects Escherichia coli and a wide range of other gram-negative enteric bacteria. Mu is a phage that replicates by transposition, which happens on induction of the quiescent phage or during lytic infection. Thus, replication of Mu phage occurs through a series of replicative transposition events. There is one novel feature of Mu that expands its host range which happens through unusual host-range expansion mechanism because of the gene of our interest, the mom gene. The gene mom stands for modification of mutator. This gene helps in modifying the DNA of the phage and acts as a saviour to the phage. The mechanism of modification is a post-replicative DNA modification. This function helps the viral genome resistant to cleavage by a wide variety of restriction endonucleases. Thus, mom gene acts as a protective sheath against nucleolytic attack when the Mu genome infects a bacterial cell possessing host specificity or RM (restriction modification) system different from the one in which the phage had been last grown. Our objective is to purify the Mom protein and investigate its function using site-directed mutagenesis with wild-type as the control.

Please feel free to contact me in case you want a more detailed report/poster for any of my research projects

Academic and Related Achievements

  • Recipient of gold medal at the prestigious Internationally Genetically Engineered Machine (iGEM) competition at the regional jamboree held at Chinese University of Hong Kong (CUHK) (2013)
  • Recipient of a grant of INR 2lakh from Industrial Consultancy & Sponsored Research (ICSR) to carry out a research project on the topic entitled “ Combating Shiga-toxin: A synthetic biology approach"
  • Recipient of the prestigious Summer Research Fellowship Program from Indian Academy of Sciences to carry out research project at Indian Institute of Science (IISc) Banagalore(2012)
  • Among top 1% in the state in STD XII & 7th rank holder in my school(2009)
  • Among top 2% to clear world's 5th toughest examination and to get admission into the prestigious Indian Institute of Technology Madras (2010)
  • Aman Kumar

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