User:Andrew K Farag/Notebook/Andrewfarag/2013/09/24: Difference between revisions
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Using C<sub>1</sub>V<sub>1</sub>=C<sub>2</sub>V<sub>2</sub>, the amount of pepsin used 0.83 uL. | Using C<sub>1</sub>V<sub>1</sub>=C<sub>2</sub>V<sub>2</sub>, the amount of pepsin used 0.83 uL. | ||
Initial concentration of pepsin is 12 uM. Final concentration is 2 nM. Final volume is 5 mL. | Initial concentration of pepsin is 12 uM. Final concentration is 2 nM. Final volume is 5 mL. | ||
< | <br> The amount of pepstatin used as well is 8.3 uL as the final concentration is to be 2uM. The initial concentration of pepstatin is 1.2 mM. | ||
<br>[[Image:Pepsin.png]] | |||
<br>[[Image:Pepstatin.png]] | |||
==Notes== | ==Notes== |
Revision as of 18:41, 13 October 2013
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ObjectiveTo observe the catalytic activity of pepsin (in cleaving peptide bonds in hemoglobin) in the presence and absence of pepstatin. This data will be compared with data we take in a later lab on pepsin-AuNPs. DescriptionThe experimental details (pepsin cleavage of hemoglobin) are similar to this reference. We will allow the pepsin cleavage of hemoglobin to proceed for two hours with aliquots taken and analyzed every 1/2 hour. We will also run parallel reactions with different concentrations of pepstatin (pepsin inhibitor) present. We will save our aliquots for analysis tomorrow using SDS-PAGE.
DataUsing C1V1=C2V2, the amount of pepsin used 0.83 uL.
Initial concentration of pepsin is 12 uM. Final concentration is 2 nM. Final volume is 5 mL.
NotesThis area is for any observations or conclusions that you would like to note.
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