User:Andrew K Farag/Notebook/Andrewfarag/2013/10/09

(Difference between revisions)
 Revision as of 15:55, 14 October 2013 (view source) (→Entry title)← Previous diff Revision as of 15:59, 14 October 2013 (view source) (→Description)Next diff → Line 21: Line 21: ==Description== ==Description== - # Add experimental record here. Include what, how, and why... + The absorbance graph is saved every 15 seconds for a period of 10 minutes total. Using the following equations, concentrations of Adenosine and Inosine with the inhibitor are calculated. +
Absorbance at 249nm=Molar absortivity of Adenosine at 249nm * concentration of Adenosine + molar absortivity of Inosine at 249 * concentration of Inosine +
Absorbance at 259nm=Molar absortivity of Adenosine at 259nm * concentration of Adenosine + molar absortivity of Inosine at 259nm * concentration of Inosine ==Data== ==Data==

Revision as of 15:59, 14 October 2013

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Objective

We are running the same procedure as yesterday only with an inhibitor, EHNA.

Description

The absorbance graph is saved every 15 seconds for a period of 10 minutes total. Using the following equations, concentrations of Adenosine and Inosine with the inhibitor are calculated.
Absorbance at 249nm=Molar absortivity of Adenosine at 249nm * concentration of Adenosine + molar absortivity of Inosine at 249 * concentration of Inosine
Absorbance at 259nm=Molar absortivity of Adenosine at 259nm * concentration of Adenosine + molar absortivity of Inosine at 259nm * concentration of Inosine

Data

EHNA stock

1. 5mg EHNA in 1mL DMSO ---> 15.9mM EHNA
2. (1.9μL)(15.9mM EHNA)=(10mL)(C2). C2 ---> 3μM EHNA

The reaction samples will contain roughly 1nM EHNA

Notes

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