User:Andrew K Farag/Notebook/Andrewfarag/2014/01/30

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
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* Insert content here...
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==objective==
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The objective for today is to begin the synthesis of BSA:L-Cysteine Nanocrystals
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==Preparation of stock solutions==
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# BSA
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#L-Cysteine
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#Sodium Hydroxide
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#Sodium Sulfide
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==Protocol BSA:L-cysteine Nanocrystals==
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# In test tube mix 180 uL of 5mM Zinc Nitrate and 20 uL of 5mM Manganese Acetate
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# Add 200 uL of 40 mM Ammonium Acetate
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# Add 200 uL of Protein:ligand in various ratios from 0% to 100% protein
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# Adjust pH to 8.2 using roughly 250 uL of 10mM Ammonium Hydroxide, and bring volume of water to 2.3 mL 
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# Incubate at room temp for 1 hour
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# Add 200 uL of 5mM Sodium Sulfide drop wise while vortexing
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# Incubate for five days at 37 degrees Celsius
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==Table of BSA:L-Cysteine ratios==
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{| {{table}}
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| mol of protein||2.00E-09||1.80E-09||1.60E-09||1.40E-09||1.20E-09||1.00E-09||8.00E-10||6.00E-10||4.00E-10||2.00E-10||0
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|-
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| percentage of protein||100||90||80||70||60||50||40||30||20||10||0
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|-
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| percentage of ligand||0||10||20||30||40||50||60||70||80||90||100
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|-
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| mol of ligand||0||2.00E-10||4.00E-10||6.00E-10||8.00E-10||1.00E-09||1.20E-09||1.40E-09||1.60E-09||1.80E-09||2.00E-09
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Revision as of 12:56, 29 January 2014

Biomaterials Design Lab Main project page
Previous entry      


objective

The objective for today is to begin the synthesis of BSA:L-Cysteine Nanocrystals

Preparation of stock solutions

  1. BSA
  2. L-Cysteine
  3. Sodium Hydroxide
  4. Sodium Sulfide

Protocol BSA:L-cysteine Nanocrystals

  1. In test tube mix 180 uL of 5mM Zinc Nitrate and 20 uL of 5mM Manganese Acetate
  2. Add 200 uL of 40 mM Ammonium Acetate
  3. Add 200 uL of Protein:ligand in various ratios from 0% to 100% protein
  4. Adjust pH to 8.2 using roughly 250 uL of 10mM Ammonium Hydroxide, and bring volume of water to 2.3 mL
  5. Incubate at room temp for 1 hour
  6. Add 200 uL of 5mM Sodium Sulfide drop wise while vortexing
  7. Incubate for five days at 37 degrees Celsius

Table of BSA:L-Cysteine ratios

mol of protein2.00E-091.80E-091.60E-091.40E-091.20E-091.00E-098.00E-106.00E-104.00E-102.00E-100
percentage of protein1009080706050403020100
percentage of ligand0102030405060708090100
mol of ligand02.00E-104.00E-106.00E-108.00E-101.00E-091.20E-091.40E-091.60E-091.80E-092.00E-09



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